2013 Fiscal Year Final Research Report
The development of a serum-free medium utilizing the interaction between growth factors and biomaterials.
| Project/Area Number |
23792273
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 無血清培地 / 軟骨細胞 / 成長因子 / ヒアルロン酸 / 生体材料 |
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| Research Abstract |
To promote clinical application of cartilage tissue engineering, we should establish a serum-free chondrocyte growth medium. We examined the combinations of growth factors and the methods to enhance their effects by making use of the interaction with biomaterials. From various growth factors that are contained within the serum, we made the cocktail of FGF-2 , insulin, EGF, PDGF and TGF-b, Moreover, we used the biomaterials including albumin and hyaluronan as the carrier of those factors. By direct mixing of those factors with biomaterials before the administration to the medium, the medium containing those mixture showed the chondrocyte growth of approximately a 25-fold increase by day 10.
Due to the optimal usage of biomaterials, this serum-free medium will realize a constant harvest of chondrocytes and could contribute to the safety and quality in regenerative medicine.
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[Journal Article] Selection of highly osteogenic and chondrogenic cells from bone marrow stromal cells in biocompatible polymer-coated plates2010
Author(s)
Liu G, Iwata K, Asawara T, Watanabe J, Fukazawa K, Ishihara K, Asawa Y, Fujihara Y, Chung UL, Moro T, Takatori Y, Takato T, Nakamura K, Kawaguchi H, Hoshi K
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Journal Title
J Biomed Mater Res A
Volume: 15;92(4)
Pages: 1273-1282
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[Presentation] Development of implant-type tissue-engineered cartilage applied for the nasal correction of the cleft lip and palate patients2011
Author(s)
Kazuto Hoshi, Yuko Fujihara, Satoru Nishizawa, Yukiyo Asawa, Sanshiro Kanazawa, Makoto Watanabe, Tomoaki Sakamoto, Ryoko Inaki, Misaki Takei, Mariko Matsuyama, Sakura Uto, Kumiko Kurabayashi, Toru Ogasawara,* Satoru Nagata, Tsuyoshi Takato
Organizer
9th European Craniofacial Congress
Place of Presentation
Salzburg, Austria
Year and Date
20110914-17
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[Presentation] DEVELOPMENT OF IMPLANT-TYPE TISSUE-ENGINEERED CARTILAGE APPLIED FOR THE CORRECTION OF THE CLEFT LIP-NOSE PATIENTS2011
Author(s)
K. Hoshi, Y. Fujihara, S. Nishizawa, Y. Asawa, S. Kanazawa, M. Watanabe, T. Sakamoto, R. Inaki, M. Takei, M. Matsuyama, S. Uto, K. Kurabayashi, M, Harai, T. Ogasawara, S. Nagata, T. Takato
Organizer
TERMIS-AP 2011
Place of Presentation
Singapore
Year and Date
20110803-05
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