2013 Fiscal Year Final Research Report
Analysis of supporting abilities for undifferentiated dental pulp stem cells by their microenvironments
| Project/Area Number |
23792304
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 歯髄幹細胞 / 細胞外環境 / 低酸素 / 造血細胞支持 |
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| Research Abstract |
The expressions of mRNA and cytokines of PDGFRalpha and Sca-1 double positive dental pulp stem cells (DPSCs) were compared to bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs). These mRNA and cytokines expressions did not show significant difference between DPSCs and BM-MSCs except Cxcl12 mRNA expression. We investigated whether proliferation and differentiation potentials of passaged DPSCs are improved by hypoxic culture. The DPSCs well maintained a proliferation and differentiation potentials with passage under the hypoxic culture condition. These results suggest that the proliferation and differentiation potentials of DPSCs were maintained in hypoxic condition. Then, hematopoietic stem/progenitor cells (HSPCs) supporting activity of DPSCs was analyzed by in vitro co-culture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOG mice. The DPSCs as well as BM-MSCs supported human cord blood-derived HSCs.
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