2014 Fiscal Year Final Research Report
Characterization of junctional complex of lymphatic endothelial cells in caner lymphangiogenesis
Project/Area Number |
23792340
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Surgical dentistry
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Research Institution | Kobe University |
Principal Investigator |
AKASHI Masaya 神戸大学, 医学部附属病院, 助教 (40597168)
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Project Period (FY) |
2011-04-28 – 2015-03-31
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Keywords | 口腔癌 / リンパ管内皮細胞 / 細胞間接着複合体 |
Outline of Final Research Achievements |
To maintain normal function, the lymphatic endothelium is regulated by cell-cell junctions. One of objectives of this study was to characterize cell junctions in lymphatic endothelial cells and to investigate the effects of the inflammatory cytokine TNF-α on altered cell-cell junctions. Cultured human dermal lymphatic endothelial cells (HDLEC) were immunostained with the tight junction marker, and adherens junction markers, VE-cadherin. In TNF-α-treated HDLEC, we evaluated changes in endothelial cell junctions by immunostaining and through the use of transendothelial electrical resistance (TER). The results revealed a heterogeneous distribution of cell junctions in HDLEC involving continuous and discontinuous junctions. Our data also suggest that TNF-α alters the normal distribution of cell junctions and affects the endothelial barrier of cultured lymphatic endothelial cells.
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Free Research Field |
口腔外科
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