2013 Fiscal Year Final Research Report
New strategy for bone regeneration based on RNA silencing of GPCR-B
| Project/Area Number |
23792421
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
WATARI IPPEI 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (10431941)
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| Project Period (FY) |
2011 – 2012
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| Keywords | GPCR class B / GLP-1 / GIP / 骨再生 / RNAサイレンシング / 唾液腺遺伝子治療 |
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| Research Abstract |
The purpose of this project is to explore the possible implementation of GPCR-B signaling in a new treatment method for bone diseases using an in vitro approach. Incretin receptors, GLP-1 receptor (GLP-1R) and GIP receptor (GIPR), belong to GPCR-B family and are suggested to play an important role in bone metabolism. The osteoblastic cell line, MC3T3-E1 was found to express both GIPR and GLP-1R. The quantitative PCR analysis showed that expression of both receptors was upregulated after stimulation with BMP-2. Determination of molecular mechanisms involved in regulation of bone metabolism by incretin receptors-related signaling will help to developed effective strategy for bone regeneration.
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