Analysis of osteoclastogenesis regulated by orthodontic force

2012 Fiscal Year Final Research Report

• Project/Area Number: 23792455
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: ARAI Atsushi 松本歯科大学, 歯学部, 助教 (00532772)
• Project Period (FY): 2011 – 2012
• Keywords: 破骨細胞 / 破骨細胞前駆細胞 / RANK / RANKL / c-Fos / M-CSF / c-fmsマクロファージ / 矯正力 / 歯の移動

Research Abstract

We previously reported that osteoclast precursors were detected as RANK-positive cells in bone tissues. RANK-positive cells were observed in bone tissues in RANKL-/- mice, but not in c-Fos-/- mice. c-Fos, but not RANKL, is required for the up-regulation of RANK in osteoclast precursors. Then, we analyzed the mechanism of c-Fos-mediated up-regulation of RANK in osteoclast precursors. Our results suggest that c-Fos plays essential roles not only in RANKL-induced formation of osteoclasts but also in M-CSF-induced formation of osteoclast precursors.

Research Products

• [Journal Article] Spleen serves as a reservoir of osteoclast precursors through vitamin D-induced IL 34 expression in osteopetrotic op/op mice. (2012)
  • Author(s): Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penniger JM, Yasuda Y, Kato S, DeLuca HF, Suda T, Udagawa N, Takahashi N
  • Journal Title: PNAS. Jun Volume: 19(109) Pages: 10006-10011
  • DOI: doi:10.1073/pnas.1207361109
  • Peer Reviewed
• [Journal Article] Fos plays an essential role in the upregulation of RANK expression in osteoclast precursors within the bone microenvironment. (2012)
  • Author(s): Arai A, Mizoguchi T, Harada S, Kobayashi Y, Nakamichi Y, Yasuda H, Penninger JM, Yamada K, Udagawa N, Takahashi N
  • Journal Title: J Cell Sci. Feb; Volume: 12(125) Pages: 2910-17
  • DOI: doi:10.1242/jcs.099986
  • Peer Reviewed
• [Journal Article] Lineage-committed osteoclast precursors circulate in blood and settle down into bone. (2011)
  • Author(s): Muto A, Mizoguchi T, Udagawa N, Ito S, Kawahara I, Abiko Y, Arai A, Harada S, Kobayashi Y, Nakamichi Y, Penninger JM, Noguchi T, Takahashi N
  • Journal Title: J Bone MinerRes.Dec; Volume: 26(12) Pages: 2978-90
  • DOI: doi:10.1002/jbmr.490
  • Peer Reviewed
• [Presentation] 2012 the American Society for Bone and Mineral Research Annual Meeting 2012 年 10月 c-Fos plays an essential role in up-regulation of RANK expression in osteoclast precursors A. Arai (2012)
  • Author(s): T. Mizoguchi , Y. Kobayashi ,T. Yamashita,K. Yamada , J. M. Penninger , N. Udagawa,N. Takahashi
  • Organizer: Journal of Bone and Mineral Research 23, suppl 1: S179,
  • Year and Date: 20120000
• [Presentation] in vivoにおける破骨細胞分化機構の解析-M-CSFはc-Fosを介し前駆細胞のRANKを上昇する (2011)
  • Author(s): 荒井敦, 溝口利英, 小林泰浩, 山 下照仁, 山田一尋, 宇田川信之, 高橋直之
  • Organizer: 松本歯科大学学会
  • Year and Date: 20110700
• [Presentation] 生体内における破骨細胞分化機構の解析-M-CSF は c-Fos を介して破骨細胞前駆細胞の RANK 発現を上昇する- (2011)
  • Author(s): 荒井 敦 , 溝口利英, 原田卓, 武藤昭紀, 小林泰浩, 山 下照仁, 保田尚孝, 山田一尋,宇田川信 之, 高橋直之
  • Organizer: 第29回日本骨代謝学会
  • Year and Date: 20110700
• [Presentation] 月c-Fos 遺伝子欠損マウスを用いた BMP 誘導 性異所性骨形成に関する解析中村みどり (2011)
  • Author(s): 二宮 禎,溝口利英,荒 井敦 , 高橋直之, 宇田川信之
  • Organizer: 第29回日本骨代謝学会
  • Year and Date: 20110700
• [Presentation] IOF Regionals 2nd Asia-Pacific Osteoporosis and Bone Meeting ANZBMS Annual Scientific Meeting, with JSBMR2011年 9月BMP-induced ectopic bone formation in c-Fos-deficient mice (2011)
  • Author(s): M. Nakamura, T. Ninomiya, T. Mizoguchi, A. Arai, N. Takahashi, N. Udagawa
  • Organizer: 第2回アジア太平洋骨粗鬆症学会プログラム抄録集
  • Year and Date: 20110000
• [Presentation] IOF Regionals 2nd Asia-Pacific Osteoporosis and Bone Meeting ANZBMS Annual Scientific Meeting, with JSBMR2011 年 9 月 c-Fos plays an essential role in up-regulation of RANK expression in osteoclast precursors (2011)
  • Author(s): A. Arai, T. Mizoguchi , Y. Kobayashi ,T.Yamashita, K. Yamada , J. M. Penninger , N. Udagawa, N. Takahashi
  • Organizer: 第2回アジア太平洋骨粗 鬆症学会プログラム抄録集
  • Year and Date: 20110000