2013 Fiscal Year Final Research Report
Identification of novel amelogenin-binding proteins by proteomics analysis
| Project/Area Number |
23792480
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
FUKUDA Takao 九州大学, 大学病院, その他 (80507781)
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| Project Period (FY) |
2011 – 2013
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| Keywords | アメロジェニン / 歯周組織 / 再生医学 / 歯学 / プロテーム |
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| Research Abstract |
Amelogenin, the major component of enamel matrix derivative (Straumann® Emdogain), is a suggested bioactive candidate for promoting the regeneration of bone and periodontal tissues. In this study, we combined affinity chromatography and proteomic analysis to characterize amelogenin-binding proteins in an osteoblast cell line. Among the identified amelogenin-interacting proteins, we validated the biological interaction of amelogenin with Grp78, which was identified in both cytosolic and membrane-enriched fractions. We demonstrated that the interaction of amelogenin with Grp78 contributed to cell proliferation, rather than correlate with the osteogenesis in SaOS-2 cells.
These findings suggest that the differential effects of amelogenin-derived osteoblast activation could be of potential clinical significance for understanding the cellular and molecular bases of amelogenin-induced periodontal tissue regeneration.
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