The possible mechanism of periodontal pathogens associated with intra-uterin infection

2014 Fiscal Year Final Research Report

• Project/Area Number: 23792483
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Kagoshima University
• Principal Investigator: HASEGAWA KOZUE 鹿児島大学, 医歯(薬)学総合研究科, 助教 (00404492)
• Co-Investigator(Renkei-kenkyūsha): NOGUCHI Kazuyuki 鹿児島大学, 医歯学総合研究科, 教授 (90218298) ; DOUCHI Tsutomu 鹿児島大学, 医歯学総合研究科, 教授 (60150413) ; NAKAMURA Toshiaki 鹿児島大学, 医歯学総合研究科, 助教 (60381183)
• Research Collaborator: HATAE Masayuki 医療法人聖成会, 柿木病院, 副院長 ; KAWAMATA Kazuya 愛育病院, 副院長 ; TATEISHI Fumi 医療法人社団忠重会 忠重歯科医院, 歯科医師
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 歯周病 / 早産・低体重児出産 / 歯周病原細菌

Outline of Final Research Achievements

F. nucleatum was detected in all oral samples and seven chorionic tissues of the high-risk pregnant women, but was not detected in chorionic tissues of the normal pregnant women. F. nucleatum LPS significantly increased IL-6 and CRH secretion by chorion-derived cells. The F. nucleatum LPS-induced IL-6 and CRH levels were significantly reduced in TLR-2 or TLR-4 gene-silenced chorion-derived cells.We suggest that F. nucleatum is detected in chorionic tissues of high-risk pregnant women, but not in chorionic tissues of normal pregnant women, and that F. nucleatum induces IL-6 and CRH production via both TLR-2 and TLR-4 in chorion-derived cells.In vivo study, P.gingivalis was detected in fetalmembrane and placenta from mice of which P.gingivalis was injected into the teil vein. The TLR-2 and TLR-4 mRNA expression were significantly increased in fetal membrane and placenta from P. gingivalis injected mice compared with those from non-infected mice.

Free Research Field

歯周病学