2012 Fiscal Year Final Research Report
Development of wound care management of wound infection based on wound infection pathogenesis
| Project/Area Number |
23792692
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Community health/Gerontological nurisng
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAGAMI Gojiro 東京大学, 大学院・医学系研究科, 講師 (70547827)
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| Project Period (FY) |
2011 – 2012
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| Keywords | クオラムセンシング / シグナル伝達 / 褥瘡 / 感染 |
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| Research Abstract |
This study aimed to establish an appropriate strategy for prevention and control of pressure ulcer infection, an unsolved problem among elderly population. For exploring mechanism responsible for pressure ulcer infection, we targeted Pseudomonas aeruginosa quorum sensing system. We demonstrated the increased expression of matrix metalloproteinase (Mmp-9) gene, which acts as an inflammatory cytokine and proteinase, in fibroblasts exposed to 3-oxo-dodecanoyl homoserine lactone (AHL). This induction was occurred via AP-1/ERK/p38 signaling pathway. For further exploring mechanism above this pathway, we assessed the gene expression changes by DNA microarray using fibroblast exposed to 10μM of AHL, which is determined to be detrimental concentration for fibroblast response to AHL. This study suggested the contribution of AHL on the inflammation and tissue destruction by P. aeruginosa during wound healing process through the specific signaling pathway to fibroblast by inducing Mmp-9 gene expression.
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Research Products
(9 results)
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[Presentation] Investigation of the influence of Pseudomonas aeruginosa quorum sensing signal molecule N-(3-oxyododecanoyl) homoserine lactone on keratinocyte migration2012
Author(s)
Paes C, Nakagami G, Minematsu T, Nagase T, Huang L, Asada M, Yamamoto Y, Kanazawa T, Kishi C, Sanada H
Organizer
The 4th Congress of the World Union of Wound Healing Societies. OR103
Place of Presentation
Yokohama, Sep
Year and Date
20120000
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[Presentation] The Pseudomonas aeruginosa quorum sensing signal molecule, N-(3-oxododecanoyl) homoserine lactone, induces matrix metalloproteinase expression via MAPK / AP-1 pathway in rat fibroblast2011
Author(s)
Nakagami G, Minematsu T, Nagase T,Morohoshi T, Ikeda T, Asada M, Paes C, Sanada H
Organizer
21st Conference of the European Wound Management Association
Place of Presentation
Brussels, Belgium, May
Year and Date
20110000
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[Presentation] In vitro effect of the Pseudomonas aeruginosa quorum sensing signal molecule, N-(3-oxododecanoyl) homoserine lactone, on keratinocyte migration2011
Author(s)
Paes C, Nakagami G, Minematsu T, Nagase T, Morohoshi T, Ikeda T, Sanada H
Organizer
21st Conference of the European Wound Management Association
Place of Presentation
Brussels, Belgium, May
Year and Date
20110000
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