Pharmacological Stimulation of Mesenchymal Stromal/Stem Cells for Umbilical Cord Blood Transplantation

2012 Fiscal Year Final Research Report

• Project/Area Number: 23890090
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Kyoto University
• Principal Investigator: MIURA Yasuo 京都大学, 大学院・医学研究科, 助教 (70605146)
• Research Collaborator: YAO Hisayuki 京都大学, 大学院・医学研究科, 研修員 ; YOSHIOKA Satoshi 京都大学, 大学院・医学研究科, 研修員
• Project Period (FY): 2011 – 2012
• Keywords: 間葉系幹細胞 / 臍帯血 / 造血細胞移植 / 細胞治療

Research Abstract

The demand of umbilical cord blood transplantation has been increased in our country with declining birth rate and aging of the population. However, engraftment failure is one of theclinical issues to be solved in umbilical cord blood transplantation. Mesenchymal stromal/stem cells have an ability to support hematopoiesis, but its frequency in umbilical cord blood is low. In this project, we found a novel and effective method to isolate mesenchymal stromal/stem cells from umbilical cord blood. Moreover, we found that the hematopoiesis-supportive ability of mesenchymal stromal/stem cells is qualitatively enhanced by the pharmacological stimulation in addition to the quantitative expansion by cell culture.

Research Products

• [Journal Article] 間葉系幹細胞と造血 (2013)
  • Author(s): 三浦康生
  • Journal Title: 血液フロンティア Volume: 23巻 Pages: 21-27
  • URL: https://www.iyaku-j.com/iyakuj/system/M2-1/summary_viewer.php?trgid=26437
• [Journal Article] C/EBPβpromotes BCR-ABL-mediated myeloid expansion and leukemic stem cell exhaustion (2013)
  • Author(s): Hayashi Y, Hirai H, Kamio N, Yao H, Yoshioka S, Miura Y, Ashihara E, Fujiyama Y, Tenen DG and Maekawa T
  • Journal Title: Leukemia Volume: 27 Pages: 619-628
  • DOI: doi:10.1038/leu.2012.258
  • Peer Reviewed
• [Journal Article] C/EBPβ is involved in the amplification of early granulocyte precursors during candidemia-induced 'emergency' granulopoiesis (2012)
  • Author(s): Satake S, Hirai H, Hayashi Y, Shime N, Tamura A, Yao H, Yoshioka S, Miura Y, Inaba T, Fujita N, Ashihara E, Imanish J, Sawa T and Maekawa T
  • Journal Title: Journal of Immunology Volume: 189 Pages: 4546-4555
  • DOI: doi:10.4049/jimmunol.1103007
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞 ～no longer second class marrow citizens～ (2011)
  • Author(s): 三浦康生
  • Journal Title: 京都がん研究会メールマガジン Volume: 87巻 Pages: 7
  • URL: http://ganpro.med.kyoto-u.ac.jp/publicity/kyoto_cancer/kyoto_cancer.htm
• [Journal Article] 間葉系幹細胞による造血制御と臨床応用
  • Author(s): 三浦康生
  • Journal Title: 血液内科 Volume: (印刷中)
  • URL: http://www.kahyo.com/category/A1-KS
• [Journal Article] 間葉系幹細胞による造血制御
  • Author(s): 三浦康生
  • Journal Title: 臨床血液 Volume: 5巻 Pages: 431-435
  • URL: https://www.jstage.jst.go.jp/browse/rinketsu/-char/ja
  • Peer Reviewed
• [Presentation] 接着法による効率的なさい帯血からの間葉系幹細胞分離に関する検討(抄録番号OS-16-2) (2013)
  • Author(s): 吉岡聡、三浦康生, et al
  • Organizer: 第61回日本輸血・細胞治療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20130516-18
• [Presentation] Pharmacological Targeting of Bone Marrow Mesenchymal Stromal/Stem cells for Regulation of Hematopoiesis (2013)
  • Author(s): Miura Y
  • Organizer: AsiaCORD 2013, Scientific Symposium I-2 "New Insights into Stem Cell Biology-Interactions between Stromal Cells & Solid tumor/Leukemic Stem Cells"
  • Place of Presentation: Kobe
  • Year and Date: 20130419-20
• [Presentation] Parathyroid hormone enhances expansion through CDH11 in human bone marrow mesenchymal stromal/stem cells (2013)
  • Author(s): Yao H, Miura Y, et al
  • Organizer: AsiaCORD 2013, Scientific SymposiumI-1 "New Insights into Stem Cell BiologyStem Cell Development & Differentiation"
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20130419-20
• [Presentation] C/EBPβ in mesenchymal stromal/stem cells regulates early B cell lymphopoiesis (2013)
  • Author(s): Yoshioka S, Miura Y, et al
  • Organizer: AsiaCORD 2013, Scientific Symposium I-1 "New Insights into Stem Cell Biology- Stem Cell Development & Differentiation"
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20130419-20
• [Presentation] Expression of C/EBPβ in bone marrow mesenchymal stem cells is mandatory for early-stage B cell lymphopoiesis [Abstract #3457] (2012)
  • Author(s): Yoshioka S, Miura Y, et al
  • Organizer: American Society of Hematology, 54th Annual Meeting
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 20121208-11
• [Presentation] Mesenchymal stem cells skewed their phenotype toward the osteogenic lineage support the hematopoietic cell differentiation [Abstract #3458] (2012)
  • Author(s): Yao H, Miura Y, et al
  • Organizer: American Society of Hematology, 54th Annual Meeting
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 20121208-11
• [Presentation] Defective mesenchymal stem cells contribute to impaired B cell lymphopoiesis in C/EBPβ deficient mice [Abstract #OS-2-25] (2012)
  • Author(s): Yoshioka S, Miura Y, et al
  • Organizer: The 74th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: Kyoto
  • Year and Date: 20121019-21
• [Presentation] Parathyroid hormone stimulates mesenchymal stem cells to enhance expansion of hematopoietic cells [Abstract # OS-1-119] (2012)
  • Author(s): Yao H, Miura Y, et al
  • Organizer: The 74th Annual Meeting of the Japanese Society of Hematology
  • Place of Presentation: Kyoto
  • Year and Date: 20121019-21
• [Presentation] Pharmacological mesenchymal stem cell-based intervention as a novel approach to expand hematopoietic cells (2012)
  • Author(s): Yao H, Miura Y, et al
  • Organizer: Kyoto University Global COE "Center for Frontier Medicine" International Symposium/Retreat 2012
  • Place of Presentation: Hyogo
  • Year and Date: 20121005-06
• [Presentation] Regulation of hematopoiesis by mesenchymal stem cells (2012)
  • Author(s): Miura Y
  • Organizer: The 74thAnnual Meeting of the Japanese Society of Hematology, Symposium 4 "Mesenchymal stem cells: Progress Toward Clinical Application for Hematopoietic Stem Cell Transplantation"
  • Place of Presentation: Kyoto
  • Year and Date: 2012-10-20
• [Presentation] 間葉系幹細胞による造血制御と臨床応用に向けた基礎的検討 (2012)
  • Author(s): 三浦康生
  • Organizer: 特定非営利活動法人HLA研究所京滋免疫血液研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-02-16
• [Presentation] Parathyroid Hormone Stimulates Human Mesenchymal Stem Cells to Enhance the Expansion of Hematopoietic Stem Cells [Abstract #3407] (2011)
  • Author(s): Yao H, Miura Y, et al
  • Organizer: American Society of Hematology, 53rd Annual Meeting
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 20111210-13