2012 Fiscal Year Final Research Report

Analysis of the mechanism of action of antipsychotics by highly sensitive quantitative proteomics

Research Project

Project/Area Number	23890200
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Psychiatric science
Research Institution	Dokkyo Medical University
Principal Investigator	ARIME Yosefu 獨協医科大学, 医学部, 助教 (80609404)
Project Period (FY)	2011 – 2012
Keywords	統合失調症 / モデルマウス / 行動 / 抗精神病薬 / 受容体占拠率 / 定量プロテオミクス
Research Abstract	The purpose of this study was to set up the experimental system of quantitative proteomics for mouse brain protein and explore the mechanism of action of antipsychotics. We found that enhanced response to an acute phencyclidine (PCP) challenge in mice previously undergoing chronic PCP treatment, called as sensitization,was significantly attenuated by treatment with clinically relevant antipsychotics dose regimen using osmotic minipump. And, we established a method of cell fractionation, peptides specific for each target protein including membrane protein and simultaneous quantification method for their proteins using LC-MS/MS.

(3 results)