Construction of regeneration technology of tooth germ utilizing control the contact between epithelial and mesenchymal using neural crest derived cells residing in hair follicle

2012 Fiscal Year Final Research Report

• Project/Area Number: 23890209
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Showa University
• Principal Investigator: MIYAUCHI Tomohiko 昭和大学, 歯学部, 助教 (20611502)
• Project Period (FY): 2011 – 2012
• Keywords: 再生医学 / 神経堤由来細胞 / 歯胚再生 / 象牙芽細胞 / エナメル芽細胞

Research Abstract

It is known that the neural crest derived cells contribute to odontoblasts of the tooth and osteoblasts in the craniofacial region. The presence of neural crest-derived cells in the facial skin and whisker pad was reported previously. In addition, some neural crest cells are maintained in an undifferentiated state as neural crest-derived stem cells throughout the life of the animal. Therefore, neural crest cells expected to be applied to regenerative medicine. The morphogenesis of hair follicle as well as tooth initiates from epithelial-mesenchymal interactions. These properties prompted us to manufacture the epithelium - mesenchymal spheroids using pattern culture that control the placement and configuration of the epithelial cells utilizing optical lithography.The purpose of this study is to analyse the ability of tooth germ formation of epithelium- mesenchymal spheroids towards the regenerative medicine of tooth.

Research Products

• [Journal Article] Identification and isolation of neural crest derived cells in nasal concha (2011)
  • Author(s): Ono M, Suzawa T, Takami M, Miyauchi T, Ogasawara A, Yamada A, Hosono T, Arata S, Miyamoto Y, Baba K, Nakamura M, Osumi N, Maki K, Kamijo R
  • Journal Title: Jpn J Tissue Cult Dent Res Volume: 20(2) Pages: 29-35
  • Peer Reviewed
• [Presentation] 成体マウス毛包内の神経堤由来細胞による象牙芽細胞分化誘導 (2012)
  • Author(s): 森澤絵里,須澤徹夫,宮内知彦,鈴木航,馬場一美,上條竜太郎
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
  • Year and Date: 20120900
• [Presentation] 毛包から採取した神経堤由来細胞による象牙芽細胞分化誘導 (2012)
  • Author(s): 森澤絵里,須澤徹夫,宮内知彦,高橋正皓,槇宏太郎,馬場一美,大隅典子,上條竜太郎
  • Organizer: 第46回日本口腔科学会関東地方部会
  • Place of Presentation: 川越
  • Year and Date: 20120900
• [Presentation] 毛包内の神経堤由来細胞による象牙芽細胞分化誘導 (2012)
  • Author(s): 森澤絵里,須澤徹夫,宮内知彦,高見正道,山田篤,宮本洋一,林竜平,西田幸二,大隅典子,馬場一美,上條竜太郎
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20120600
• [Presentation] 再生医療における神経堤細胞の可能性 (2012)
  • Author(s): 宮内知彦,須澤徹夫,森澤絵里,大隅典子,上條竜太郎,馬場一美
  • Organizer: 口腔先端応用医科学研究会 第4回学術会議
  • Place of Presentation: 東京
  • Year and Date: 20120100
• [Presentation] 象牙質再生に向けた毛包神経堤細胞による象牙芽細胞分化誘導 (2011)
  • Author(s): 宮内知彦,須澤徹夫,上條竜太郎,馬場一美
  • Organizer: 第41回日本口腔インプラント学会学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 20110900
• [Presentation] 口腔・顎顔面部に存在する神経堤由来細胞を用いた硬組織再生法の確立 (2011)
  • Author(s): 須澤徹夫,宮内知彦,小野美樹,高見正道,槇宏太郎,馬場一美,大隅典子,上條竜太郎
  • Organizer: 第11回東京骨関節フォーラム
  • Place of Presentation: 東京
  • Year and Date: 20110800
• [Presentation] 成体における神経堤由来細胞の分布とそれを用いた骨芽細胞の分化誘導 (2011)
  • Author(s): 小野美樹,高見正道,須澤徹夫,山田篤,宮内知彦,馬場一美,中村雅典,槇宏太郎,上條竜太郎
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20110700
• [Presentation] 毛包から純化した神経堤細胞の象牙芽細胞への分化誘導 (2011)
  • Author(s): 宮内知彦,須澤徹夫,小野美樹,林竜平,西田幸二,槇宏太郎,上條竜太郎,馬場一美
  • Organizer: 日本補綴歯科学会 第120回記念学術大会
  • Place of Presentation: 広島
  • Year and Date: 20110500