2012 Fiscal Year Final Research Report
Isolation and culture of c-kit-positive human cardiac stem cells (CSCs): Aiming for the first clinical trial in Japan
| Project/Area Number |
23890211
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Tokai University |
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Principal Investigator |
HOSODA Toru 東海大学, 創造科学技術研究機構, 准教授 (50608601)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 再生医学 / 体性幹細胞 / 心臓幹細胞 |
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| Research Abstract |
In order to prepare for a potential domestic clinical trial, adult and pediatric heart tissues resected during cardiac surgeries were obtained. I have succeeded in reproducing the methods to isolate and culture c-kit-positive human cardiac stem cells. Additionally, immunostaining against c-kit was carried out to confirm the specificity of the sorting procedure. Moreover, population doubling time, BrdU positivity, clonogenicity of the stem cells were examined. These data are compared with clinical information to retrieve correlations.
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Research Products
(38 results)
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[Journal Article] Cardiomyogenesis in the aging and failing human heart2012
Author(s)
Kajstura J, Rota M, Cappetta D, Ogorek B, Arranto C, Bai Y, Ferreira-Martins J, Signore S, Sanada F, Matsuda A, Kostyla J, Caballero MV, Fiorini C, D'Alessandro DA, Michler RE, Del Monte F, Hosoda T, Perrella MA, Leri A, Buchholz BA, Loscalzo J, Anversa
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Journal Title
Circulation
Volume: 126
Pages: 1869-81
DOI
Peer Reviewed
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[Journal Article] Tracking chromatid segregation to identify human cardiac stem cells that regenerate extensively the infarcted myocardium2012
Author(s)
Kajstura J, Bai Y, Cappetta D, Kim J, Arranto C, Sanada F, D'Amario D, Matsuda A, Bardelli S, Ferreira-Martins J, Hosoda T, Leri A, Rota M, Loscalzo J, Anversa P
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Journal Title
Circ Res
Volume: 111
Pages: 894-906
DOI
Peer Reviewed
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[Journal Article] Cardiomyogenesis in the developing heart is regulated by c-kit-positive cardiac stem cells2012
Author(s)
Ferreira-Martins J, Ogorek B, Cappetta D, Matsuda A, Signore S, D'Amario D, Kostyla J, Steadman E, Ide-Iwata N, Sanada F, Iaffaldano G, Ottolenghi S, Hosoda T, Anversa P, Leri A, Kajstura J, Rota M
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Journal Title
Circ Res
Volume: 110
Pages: 701-15
DOI
Peer Reviewed
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[Journal Article] Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomized phase 1 trial2011
Author(s)
Bolli R, Chugh AR, D'Amario D, Loughran JH, Stoddard MF, Ikram S, Beache GM, Wagner SG, Leri A, Hosoda T, Sanada F, Elmore JB, Goichberg P, Cappetta D, Solankhi NK, Fahsah I, Rokosh DG, Slaughter MS, Kajstura J, Anversa P
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Journal Title
Lancet
Volume: 378
Pages: 1847-57
DOI
Peer Reviewed
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[Journal Article] Response to letter regarding article, "Human cardiac stem cell differentiation is regulated by a mircrine mechanism"2011
Author(s)
Hosoda T, Zheng H, Cabral-da-Silva MC,Sanada F, Ide-Iwata N, Ogorek B, Ferreira-Martins J, Arranto C, D'Amario D, Del Monte F, Urbanek K, D'Alessandro DA, Michler RE, Anversa P, Rota M, Kajstura J, Leri A
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Journal Title
Circulation
Volume: 124
Pages: e457
DOI
Peer Reviewed
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[Journal Article] Effects of age and heart failure on human cardiac stem cell function2011
Author(s)
Cesselli D, Beltrami AP, D'Aurizio F, Marcon P, Bergamin N, Toffoletto B, Pandolfi M, Puppato E, Marino L, Signore S, Livi U, Verardo R, Piazza S, Marchionni L, Fiorini C, Schneider C, Hosoda T, Rota M, Kajstura J, Anversa P, Beltrami CA, Leri A
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Journal Title
Am J Pathol
Volume: 179
Pages: 349-66
DOI
Peer Reviewed
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[Journal Article] Insulin-like growth factor-1 receptor identifies a pool of human cardiac stem cells with superior therapeutic potential for myocardial regeneration.2011
Author(s)
D'Amario D, Cabral-Da-Silva MC, Zheng H, Fiorini C, Goichberg P, Steadman E, Ferreira-Martins J, Sanada F, Piccoli M, Cappetta D, D'Alessandro DA, Michler RE, Hosoda T, Anastasia L, Rota M, Leri A, Anversa P, Kajstura J
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Journal Title
Circ Res
Volume: 108
Pages: 1467-81
DOI
Peer Reviewed
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