2012 Fiscal Year Final Research Report
Development of novel cell detection system targeting microRNAexpression
| Project/Area Number |
23890226
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
YAMADA Hiroya 藤田保健衛生大学, 医学部, 助教 (80610352)
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| Project Period (FY) |
2011 – 2012
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| Keywords | フローサイトメトリ / 細胞検出法 / microRNA / 遺伝子検査 / mRNA quantification after FACS / FACS-mQ |
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| Research Abstract |
Recently, we have established an in-tube in situ hybridization method named mRNA quantification after fluorescence activated cell sorting (FACS-mQ), in which a specific mRNA in a particular cell type is stained with a florescent dye, allowing the stained cells to be selected by FACS without suffering excessive RNA degradation. In this study, we tried to apply this method to micro RNA detecting in the various cells.
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