2023 Fiscal Year Research-status Report
Construction of 3D hierarchical vascular networks in a cm-sized liver tissue using magnetic fields
| Project/Area Number |
23K13291
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| Research Institution | Nagoya University |
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Principal Investigator |
金 恩恵 名古屋大学, 未来社会創造機構, 研究員 (30972517)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Keywords | Micromanipulation / Magntic tweezer / Vascular network / Liver tissue |
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| Outline of Annual Research Achievements |
In the first year, we designed magnetic tweezers for constructing 3D hierarchical vascular networks in a cm-sized liver tissue. In addition, we fabricated several patterns of vascular network in cellular structure in order to compare the efficiency of the vascular networks. For this, we fabricated several patterns of vascular networks; without vascular network, with vertical channel, and with 3D hierarchical channels. After 3 weeks, the cell viability of four samples were analyzed and we found that the tissue having 3D hierarchical channels show higher cell viability than others. From the result, we can see that the fabricated 3D hierarchical channels can supply the nutrient and oxygen to the cells in tissues and improve the cell condition after a long-term culture..
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
In accordance with the original research plan, we designed the magnetic tweezers and verified the efficiency of channel networks by comparing tissue haing several patterns of vascular networks.
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| Strategy for Future Research Activity |
In the next step, we will apply perfusable network within 3D cellular structure by adding circulation system and then to transform from the empty channels to vascular network, I will seed human umbilical vein endothelial cells (HUVECs) to the channels and perfuse the culture medium to the vascular networks. Finally, we will analyze cell condition and liver function of constructed tissue.
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Research Products
(1 results)
