高次脳領域におけるシナプス伝達制御機構の分子形態学的研究

2014 Fiscal Year Annual Research Report

• Project/Area Number: 24220007
• Research Institution: Hokkaido University
• Principal Investigator: 渡辺 雅彦 北海道大学, 医学(系)研究科(研究院), 教授 (70210945)
• Co-Investigator(Kenkyū-buntansha): 崎村 建司 新潟大学, 脳研究所, 教授 (40162325)
• Project Period (FY): 2012-05-31 – 2017-03-31
• Keywords: シナプス / 情報伝達 / AMPA受容体 / 海馬 / 線条体

Outline of Annual Research Achievements

平成26年度における研究実施により、以下の成果が得られた。
海馬シナプスにおけるAMPAR発現には、顕著な入力選択性と標的選択性が存在する。この選択的発現にはTARPg-2の選択的発現が関与し、その分子のノックアウトにより入力選択性と標的選択性が失われた。対照的に、TARPg-8のシナプス発現には選択性が乏しく、そのノックアウトにより海馬シナプスの基盤的AMPAR発現が低下した。
線条体の主要なニューロンである中型有棘細胞は、皮質由来のVGluT1陽性シナプスと視床由来のVGluT2陽性シナプスを形成する。この両者のシナプスにおけるAMPARの入力選択性は無いため、その原因を追求した。その結果、海馬とは異なり、線条体ではARPg-2発現の入力選択性はないことが判明した。
黒質のドーパミン投射線維は線条体中型有棘細胞に対して対称性シナプスを形成することが知られている。このシナプス伝達に関わる分子の発現を追求した所、シナプス前部はドーパミンの合成と放出・再取り込みに関わるドーパミン作動性を有するのに対して、シナプス後部はGABA作動性でGABAの伝達のGABAシナプスの接着分子を発現していた。この接着分子のノックダウンによりドーパミンシナプスは有意に減少した。一方、ドーパミン受容体は中型有棘細胞のシナプス外領域に広く発現していた。よって、ドーパミン投射線維はGABA作動性シナプスの接着分子を利用して係留接着を行ない、これを起点としてボリューム伝達に標的選択性を与えていることが考えられた。
扁桃体基底核には、内在性カンナビノイド伝達分子が高密度に集積し、陥入型シナプスを形成している。今回、VGluT3を発現するCCK陽性バスケット細胞がこのシナプスを選択的に形成することを明らかにした。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

当初の研究計画の項目それぞれについて、着実にデータを集積し、一部は論文投稿の段階に達しているため。

Strategy for Future Research Activity

当初予定した研究計画を着実に実行し推進する。

Research Products

• [Journal Article] Is D-aspartate produced by glutamic-oxaloacetic transaminase -1 like 1(Got1l1), a putative aspartate racemase? (2015)
  • Author(s): Tanaka-Hayashi A, Hayashi S, Inoue R, Ito T, Konno K, Yoshida T, Watanabe M, Yoshimura T, Mori H
  • Journal Title: Amino Acids Volume: 47 Pages: 79-86
  • DOI: 10.1007/s00726-014-1847-3.
  • Peer Reviewed
• [Journal Article] Increased regional gray matter volume in μ-opioid receptor knockout (MOR-KO) mice as determined by MRI voxel-based morphometry. (2015)
  • Author(s): Sasaki K, Sumiyoshi A, Nonaka H, Kasahara Y, Ikeda K, Hall FS, Uhl GR, Watanabe M, Kawashima R, Sora I
  • Journal Title: Br J Pharmacol. Volume: 172 Pages: 654-667
  • DOI: 10.1111/bph.12807.
  • Peer Reviewed
• [Journal Article] Chemical corrector treatment ameliorates increased seizure susceptibility in a mouse model of familial temporal lobe epilepsy. (2015)
  • Author(s): Yokoi N, Fukata Y, Kase D, Miyazaki T, Jaegle M, Ohkawa T, Takahashi N, Iwanari H, Mochizuki Y, Hamakubo T, Imoto K, Meijer D, Watanabe M, Fukata M
  • Journal Title: Nat Med, Volume: 21 Pages: 19-26
  • DOI: 10.1038/nm.3759
  • Peer Reviewed
• [Journal Article] Distinct subsynaptic localization of type 1 metabotropic glutamate (mGlu1) receptors at glutamatergic and GABAergic synapses in the rodent cerebellar cortex. (2015)
  • Author(s): Mansouri M, Kasugai Y, Fukazawa Y, Bertaso F, Raynaud F, Perroy J, Fagni L, Kaufmann WA, Watanabe M, Shigemoto R, Ferraguti F
  • Journal Title: Eur J Neurosci, Volume: 41 Pages: 157-167
  • DOI: doi: 10.1111/ejn.12779. Epub 2014 Nov 6.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regulatory effect of interleukin-4 in the innate inflammatory response to rhodococcus aurantiacus infection in mice. (2015)
  • Author(s): Yimin, Kohanawa M, Zhao S, Li M, Kuge Y, Tamaki N, Watanabe M
  • Journal Title: J Interferon Cytokine Res. Volume: 35 Pages: 222-231
  • DOI: 10.1089/jir.2014.0107.
  • Peer Reviewed
• [Journal Article] Cell type-specific STORM superresolution imaging reveals nanoscale organization of cannabinoid signaling at hippocampal GABAergic synapses. (2015)
  • Author(s): Dudok , Barn L, Ledri M, Szabó SI, Szabadits E, Pintér B, Woodhams SG, Henstridge CM, Balla GY, Nyilas R, Vargav C, Lee S, Matolcsi M, Cervenak J, Kacskovics I, Watanabe M, Sagheddu C, Melis M, Pistis M, Soltesz I, Katona I
  • Journal Title: Nat Neurosci, Volume: 18 Pages: 75-86
  • DOI: 10.1038/nn.3892
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Short-chain fatty acid sensing in rat duodenum. (2015)
  • Author(s): Akiba Y, Inoue T, Kaji I, Higashiyama M, Iwamoto K, Watanabe M, Guth PH, Engel E, Kuwahara A, Kaunitz JD
  • Journal Title: J Physiol, Volume: 593 Pages: 585-599
  • DOI: 10.1113/jphysiol.2014.280792
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Untangling dopamine-adenosine receptor assembly in experimental parkinsonism. (2015)
  • Author(s): Fernández-Dueñas V, Taura JJ, Cottet M, Gómez-Soler M, López-Cano M, Ledent C, Watanabe M, Trinquet E, Pin JP, Luján R, Durroux T, Ciruela F
  • Journal Title: Dis Model Mech, Volume: 8 Pages: 57-63
  • DOI: 10.1242/dmm.018143
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SCFA transport in rat duodenum. (2015)
  • Author(s): Kaji I, Iwanaga T, Watanabe M, Guth P, Engel E, Kaunitz J, Akiba Y
  • Journal Title: Am J Physiol Gastrointest Liver Physiol, Volume: 308 Pages: G188-197
  • DOI: 10.1152/ajpgi.00298.2014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Structure-function relationshipsbetween aldolase C/zebrin II expression and complex spike synchrony in the cerebellum. (2015)
  • Author(s): Tsutsumi S, Yamazaki M, Miyazaki M, Watanabe M, Sakimura K, Kano M, Kitamura K
  • Journal Title: J Neurosci, Volume: 35 Pages: 843-852
  • DOI: 10.1523/JNEUROSCI.2170-14.2015.
  • Peer Reviewed
• [Journal Article] Developmental tightening of cerebellar cortical synaptic influx-release coupling. (2015)
  • Author(s): Baur D, Bornschein G, Althof D, Watanabe M, Kulik A, Eilers J, Schmidt H
  • Journal Title: J Neurosci, Volume: 35 Pages: 1858-1871
  • DOI: 10.1523/JNEUROSCI.2900-14.2015.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Anterograde C1ql1 signaling is required in order to determine and maintain a single-winner climbing fiber in the mouse cerebellum. (2015)
  • Author(s): Kakegawa W, Mitakidis N, Miura E, Abe M, Matsuda K, Takeo YH, Kohda K, Motohashi J, Takahashi A, Nagao S, Muramatsu S, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M
  • Journal Title: Neuron Volume: 85 Pages: 316-329
  • DOI: 10.1016/j.neuron.2014.12.020.
  • Peer Reviewed
• [Journal Article] VGluT3-expressing CCK-positive basket cells construct invaginating synapses enriched with endocannabinoid signaling proteins in particular cortical and cortex-like amygdaloid regions of mouse brains. (2015)
  • Author(s): Omiya Y, Uchigashima M, Konno K, Yamasaki M, Miyazaki T, Yoshida T, Kusumi I, Watanabe M
  • Journal Title: J Neurosci, Volume: 35 Pages: 4215-4225
  • DOI: 10.1523/JNEUROSCI.4681-14.2015.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice. (2015)
  • Author(s): Konno K, Takahashi-Iwanaga H, Uchigashima M, Miyasaka K, Funakoshi A, Watanabe M, Iwanaga T
  • Journal Title: Histochem Cell Biol. Volume: 143 Pages: 301-312
  • DOI: 10.1007/s00418-014-1281-3.
  • Peer Reviewed
• [Journal Article] The ascending median raphe projections are mainly glutamatergic in the mouse forebrain. (2015)
  • Author(s): Szonyi A, Mayer MI, Cserép C, Takács VT, Watanabe M, Freund TF, Nyiri G
  • Journal Title: Brain Struct Funct Volume: in press Pages: in press
  • Peer Reviewed
• [Journal Article] Involvement of diacylglycerol kinase B in the spine formation at distal dendrites of striatal medium spiny neurons. (2015)
  • Author(s): Hozumi Y, Kakefuda K, Yamasaki M, Watanabe M, Hara H, Goto K
  • Journal Title: Brain Res, Volume: 1594 Pages: 36-45
  • DOI: doi: 10.1016/j.brainres.2014.11.012. Epub 2014 Nov 15.
  • Peer Reviewed
• [Journal Article] Inhibitory interneurons that express GFP in the PrP-GFP mouse spinal cord are morphologically heterogeneous, innervated by several classes of primary afferent and include lamina I projection neurons among their postsynaptic targets. (2015)
  • Author(s): Ganley R, Iwagaki N, del Rio P, Baseer N, Dickie AC, Polgár E, Watanabe M, Abraira VE, Ginty DD, Riddell J, Todd AJ
  • Journal Title: J Neurosci Volume: in press Pages: in press
  • Peer Reviewed
• [Journal Article] Retrograde signaling for climbing fiber synapse elimination. (2015)
  • Author(s): Uesaka N, Uchigashima M, Mikuni T, Hirai H, Watanabe M, Kano M
  • Journal Title: Cerebellum Volume: 14 Pages: 4-7
  • DOI: doi: 10.1007/s12311-014-0615-y.
  • Peer Reviewed
• [Journal Article] Differential distribution of phospholipase CB isoforms and diaglycerol kinase B; in the rodents cerebella corroborates the division of the unipolar brush cell into 2 major subtypes. (2014)
  • Author(s): 1.Sekerková G, Watanabe M, Martina M, Mugnaini E
  • Journal Title: Brain Struct Funct Volume: 219 Pages: 719-744
  • DOI: DOI 10.1007/s00429-013-0531-9
  • Peer Reviewed
• [Journal Article] Dlx1 transcription factor regulates dendritic growth and postsynaptic differentiation through inhibition of neuropilin-2 and PAK3 expression. (2014)
  • Author(s): Dai X, Iwasaki H, Watanabe M, Okabe S
  • Journal Title: Eur J Neurosci, Volume: 39 Pages: 531-547
  • DOI: doi: 10.1111/ejn.12413. Epub 2013 Nov 15.
  • Peer Reviewed
• [Journal Article] GABAergic neurons in the ventral tegmental area receive dual GABA/enkephalin-mediated inhibitory inputs from the bed nucleus of the stria terminalis. (2014)
  • Author(s): Kudo T, Konno K, Uchigashima M, Yanagawa Y, Sora I, Minami M, Watanabe M
  • Journal Title: Eur. J. Neurosci. Volume: 39 Pages: 1796-1809
  • DOI: doi: 10.1111/ejn.12503. Epub 2014 Mar 2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Multiple Mechanistically Distinct Modes of Endocannabinoid Mobilization at Central Amygdala Glutamatergic Synapses. (2014)
  • Author(s): Ramikie TS, Nyilas R, Hartley ND, Gamble-George JC, Mackie K, Watanabe M, Katona I, Patel S
  • Journal Title: Neuron Volume: 81 Pages: 1111-1125,
  • DOI: doi: 10.1016/j.neuron.2014.01.012.
  • Peer Reviewed
• [Journal Article] The synaptic targeting of mGluR1 by its carboxyl-terminal domain is crucial for cerebellar function. (2014)
  • Author(s): Ohtani Y, Miyata M, Hashimoto K, Tabata T, Kishimoto Y, Fukaya M, Kase D, Kassai H, Nakao K, Hirata T, Watanabe M, Kano M, Aiba A
  • Journal Title: J Neurosci, Volume: 34 Pages: 2702-2712
  • DOI: doi: 10.1523/JNEUROSCI.3542-13.2014.
  • Peer Reviewed
• [Journal Article] A putative relay circuit providing low-threshold mechanoreceptive input to lamina I projection neurons via vertical cells in lamina II of the rat dorsal horn. (2014)
  • Author(s): Yasaka T, Tiong SYX, Polgar E, Watanabe M, Kumamoto E, Riddell JS, Todd AJ
  • Journal Title: Mol Pain Volume: 10 Pages: 3-3
  • DOI: doi: 10.1186/1744-8069-10-3.
  • Peer Reviewed
• [Journal Article] Dynorphin acts as a neuromodulator to inhibit itch in the dorsal horn of the spinal cord. (2014)
  • Author(s): Kardon A, Polgár E, Hachisuka J, Cameron D, Savage S, McClement L, Cai X, Karnup S, Fan C, Hemenway G, Bernard C, Schwartz ES, Nagase H, Watanabe M, Furuta T, Kaneko T, Todd AJ, Ross SE
  • Journal Title: Neuron Volume: 82 Pages: 573-586
  • DOI: doi: 10.1016/j.neuron.2014.02.046. Epub 2014 Apr 10.
  • Peer Reviewed
• [Journal Article] Neuronal calcium binding proteins 1/2 localize to dorsal root ganglia and excitatory spinal neurons and are regulated by nerve injury. (2014)
  • Author(s): Zhang M, Tortoriello G, Hsueh B, Tomer R, Ye L, Mitsios N, Borgius L, Kiehn O, Watanabe M, Uhlén M, Mulder J, Deisseroth K, Harkany T, Hökfelt T
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 111 Pages: E1149-1158
  • DOI: doi: 10.1073/pnas.1402318111. Epub 2014 Mar 10.
  • Peer Reviewed
• [Journal Article] Enriched expression of GluD1 in higher brain regions and its involvement in parallel fiber-interneuron synapse formation in the cerebellum. (2014)
  • Author(s): Konno K, Matsuda K, Nakamoto C, Uchigashima M, Miyazaki T, Yamasaki M, Sakimura K, Yuzaki M, Watanabe M
  • Journal Title: J. Neurosci, Volume: 34 Pages: 7412-7424
  • DOI: doi: 10.1523/JNEUROSCI.0628-14.2014.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Retrograde semaphorin signaling regulates synapse elimination in the developing mouse brain. (2014)
  • Author(s): Uesaka N, Uchigashima,M, Mikuni T, Nakazawa T, Nakao H, Hirai K, Aiba A, Watanabe M, Kano M
  • Journal Title: Science Volume: 344 Pages: 1020-1023
  • DOI: doi: 10.1126/science.1252514. Epub 2014 May 15.
  • Peer Reviewed
• [Journal Article] Neuron type- and input pathway-dependent expression of Slc4a10 in adult mouse brains. (2014)
  • Author(s): Song X, Yamasaki M, Miyazaki T, Konno K, Uchigashima M, Watanabe M
  • Journal Title: Eur J Neurosci, Volume: 40 Pages: 2797-2810
  • DOI: doi: 10.1111/ejn.12636. Epub 2014 Jun 6.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Localization of serine racemase and its role in the skin. (2014)
  • Author(s): Inoue R, Yoshihisa Y, Tojo Y, Okamura C, Yashida Y, Kishimoto J, Luan X, Watanabe M, Mizuguchi M, Nabeshima Y, Hamase K, Matsunaga K, Shimizu T, Mori H
  • Journal Title: J Invest Dermatol Volume: 134 Pages: 1618-1624, 2014
  • DOI: doi: 10.1038/jid.2014.22. Epub 2014 Jan 17.
  • Peer Reviewed
• [Journal Article] Opposing role of NMDA receptor GluN2B and GluN2D in somatosensory development and maturation. (2014)
  • Author(s): Yamasaki M, Okada R, Takasaki C, Toki S, Fukaya M, Natsume R, Sakimura K, Mishina M, Shirakawa T, Watanabe M
  • Journal Title: J Neurosci, Volume: 34 Pages: 11534-11548
  • DOI: doi: 10.1523/JNEUROSCI.1811-14.2014.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The recurrent case for the Renshaw cell. (2014)
  • Author(s): Bhumbra G, Bannatyne A, Watanabe M, Todd AJ, Maxwell D, Beato M
  • Journal Title: J Neurosci, Volume: 34 Pages: 12919-12932
  • DOI: doi: 10.1111/ejn.12470.
  • Peer Reviewed
• [Journal Article] Heterogeneous presynaptic distribution of monoacylglycerol lipase, a multipotent regulator of nociceptive circuits in the mouse spinal cord. (2014)
  • Author(s): Horváth E, Woodhams SG, Nyilas R, Henstridge CM, Kano M, Sakimura K, Watanabe M, Katona I
  • Journal Title: Eur J Neurosci, Volume: 39 Pages: 419-434
  • DOI: doi: 10.1016/j.celrep.2014.07.014. Epub 2014 Aug 7.
  • Peer Reviewed
• [Journal Article] Global scaling-down of excitatory postsynaptic responses in cerebellar Purkinje cells impairs developmental synapse elimination. (2014)
  • Author(s): Kawata S, Miyazaki T, Yamazaki M, Mikuni T, Yamasaki M, Hashimoto K, Watanabe M, Sakimura K, Kano M
  • Journal Title: Cell Rep Volume: 8 Pages: 1119-1129
  • DOI: doi: 10.1523/JNEUROSCI.2038-14.2014.
  • Peer Reviewed
• [Journal Article] Co-assembly and coupling of SK2 channels and mGlu5 receptors. (2014)
  • Author(s): Garcia-Negrego G, Soto D, Llorente J, Morató X, Galenkamp K, Gómez-Soler M, Fernández-Dueñas V, Watanabe M, Adelman J, Shigemoto R, Fukozawa Y, Luján R, Ciruela F
  • Journal Title: J Neurosci, Volume: 34 Pages: 14793-14802
  • DOI: doi: 10.1016/j.pain.2014.08.023. Epub 2014 Aug 26.
  • Peer Reviewed
• [Journal Article] Selective innervation of NK1 receptor-lacking lamina I spinoparabrachial neurons by presumed nonpeptidergic Aδ nociceptors in the rat. (2014)
  • Author(s): Baseer N, Al-Baloushi AS, Watanabe M, Shehab SA, Todd AJ
  • Journal Title: Pain Volume: 155 Pages: 2291-2300
  • DOI: doi: 10.1016/j.pain.2014.08.023. Epub 2014 Aug 26.
  • Peer Reviewed
• [Journal Article] The glutamate receptor GluN2 subunit regulates synaptic trafficking of AMPA receptors in the neonatal mouse brain. (2014)
  • Author(s): Hamada S, Ogawa I, Yamasaki M, Kiyama Y, Kassai H, Watabe AM, Nakao K, Aiba A, Watanabe M, Manabe T
  • Journal Title: Eur J Neurosci, Volume: 40 Pages: 3136-3146
  • DOI: doi: 10.1111/ejn.12682. Epub 2014 Aug 8.
  • Peer Reviewed
• [Journal Article] A new mouse allele of glutamate receptor delta 2 with cerebellar atrophy and progressive ataxia. (2014)
  • Author(s): Miyoshi Y, Yoshioka Y, Suzuki K, Miyazaki T, Koura M, Saigoh K, Kajimura K, Monobe Y, Kusunok S, Matsuda J, Watanabe M, Hayasaka N
  • Journal Title: PLoS ONE Volume: 9 Pages: e107867-e107867
  • DOI: doi: 10.1371/journal.pone.0107867. eCollection 2014.
  • Peer Reviewed
• [Journal Article] Cerebellar Plasticity and Motor Learning Deficits in a Copy Number Variation Mouse Model of Autism. (2014)
  • Author(s): Piochon C, Kloth A, Grasselli G, Titley H, Nakayama H, Hashimoto K, Wan V, Simmons D, Eissa T, Nakatani J, ACherskov A, Miyazaki T, Watanabe M, Takumi T, Kano M, Wang S, Hansel C
  • Journal Title: Nat Commun Volume: 5 Pages: 558-558
  • DOI: doi: 10.1038/ncomms6586.
  • Peer Reviewed
• [Journal Article] Expression of gastrin-releasing peptide by excitatory interneurons in the mouse superficial dorsal horn. (2014)
  • Author(s): Guttierez-Mecinas M, Watanabe M, Todd AJ
  • Journal Title: Mol Pain Volume: 10 Pages: 79-79
  • DOI: doi: 10.1186/1744-8069-10-79.
  • Peer Reviewed
• [Journal Article] Rab11a is required for apical protein localisation in the intestine. (2014)
  • Author(s): Sobajima T, Yoshimura S, Iwano T, Kunii M, Watanabe M, Atik N, Mushiake S, Morii E, Koyama Y, Miyoshi E, Harada A
  • Journal Title: Biol Open Volume: 4 Pages: 86-94
  • DOI: doi: 10.1242/bio.20148532.
  • Peer Reviewed
• [Presentation] Morphological and Physiological Approaches to Synaptic Transmission. (2015)
  • Author(s): Mochida S and Watanabe M
  • Organizer: Japan-Korea Joint Symposium: 120th Meeting of Japanese Association of Anatomists
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Cellular and molecular mechanisms for synaptic circuit pruning and plasticity. (2015)
  • Author(s): Watanabe M
  • Organizer: 120th Meeting of Japanese Association of Anatomists
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Input-selective expression of glutamate receptor GluD1 at ascending somatosensory pathway synapses in the ventral posteromedial thalamic nucleus. (2015)
  • Author(s): Kohtarou Konno, Koji Nishikawa, Masahiko Watanabe.
  • Organizer: 第92回日本生理学会・第120回日本解剖学会・全国学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Calcineurin B1 subunit expressed in Purkinje cell is essential for the formation of excitatory and inhibitory cerebellar neuronal network. (2015)
  • Author(s): Taisuke Miyazaki, Kenji Sakimura , Masahiko Watanabe
  • Organizer: 第92回日本生理学会・第120回日本解剖学会・全国学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Opposing role of NMDA receptor GluN2B and GluN2D in somatosensory development and maturation. (2015)
  • Author(s): Yamasaki M, Okada R, Takasaki C, Toki S, Fukaya M, Natsume R, Sakimura K, Mishina M, Shirakawa T, Watanabe M.
  • Organizer: 第92回日本生理学会・第120回日本解剖学会・全国学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 海馬におけるグルタミン酸受容体GluD1のシナプス形成作用. (2015)
  • Author(s): 今野幸太郎、柚崎通介、渡辺雅彦.
  • Organizer: 第20回グリアクラブ
  • Place of Presentation: KKR函館（北海道・函館市）
  • Year and Date: 2015-01-26 – 2015-01-28
• [Presentation] 皮質系領域における内因性カンナビノイド関連分子を豊富に備えた“陥入型”シナプスの分布則 (2015)
  • Author(s): 内ヶ島 基政
  • Organizer: 第20回グリアクラブ
  • Place of Presentation: KKR函館（北海道・函館市）
  • Year and Date: 2015-01-26 – 2015-01-28
• [Presentation] Enriched expression of GluD1 in higher brain regions and its involvement in parallel fiber-interneuron synapse formation in the cerebellum. (2014)
  • Author(s): Kohtarou Konno, Keiko Matsuda, Chihiro Nakamoto, Kenji Sakimura, MichisukeYuzaki, Masahiko Watanabe.
  • Organizer: Neuroscience 2014 Annual Meeting
  • Place of Presentation: Washington DC (USA)
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] mGluR1 sculpts heterologous inputs to cerebellar Purkinje cells. (2014)
  • Author(s): Watanabe M, Ichikawa R
  • Organizer: 8th International Meeting on Metabotropic Glutamate Receptors.
  • Place of Presentation: Sicily (Italy)
  • Year and Date: 2014-09-28 – 2014-10-03
• [Presentation] CCK/VGluT3陽性バスケット細胞シナプスに特異的な2-アラキドノイルグリセロールシグナルを介したシナプス伝達修飾の分子形態基盤 (2014)
  • Author(s): 内ヶ島 基政、渡辺 雅彦
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Selective expression ofglutamate receptor GluD1 at ascending pathway synapses in thalamic neurons of the ventralposteromedial nucleus. (2014)
  • Author(s): Kohtarou Konno, Koji Nishikawa, Masahiko Watanabe.
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Book] Immunocytochemistry and Related Techniques. Chapter 16. Combined immunocytochemistry and tracing of neural connections. (2014)
  • Author(s): Taisuke Miyazaki and Masahiko Watanabe
  • Total Pages: 299-312
  • Publisher: Humana Press.