2015 Fiscal Year Final Research Report
Development of new methods for a gene-manipulated rat for the analysis of human brain function
Project/Area Number |
24240048
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Neuroscience in general
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Research Institution | Niigata University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
Abe Manabu 新潟大学, 脳研究所, 准教授 (10334674)
Natsume Rie 新潟大学, 脳研究所, 技術職員 (60467082)
Nakatsukasa Ena 新潟大学, 脳研究所, 助教 (60641579)
Zhou Li 新潟大学, 脳研究所, 特任助教 (80568410)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Keywords | ラットES細胞 / 遺伝子組換え / 異種動物間キメラ / ノックアウトラット / 顕微授精 |
Outline of Final Research Achievements |
The aim of this project is to develop an efficient and low-cost method to generate gene-manipulated rats for the analysis of brain functions, so that we can provide the research community of this country with gene-modified rats as useful animal resources. For this purpose, we established an optimum culture condition for rat ES cells, a rapid gene-manipulated method using homologous recombination, and a generation method of chimeras that efficiently differentiate to germ cells. Thus, we succeeded in stable generation of gene-manipulated rats. In addition, in order to produce rat sperm from the rat ES cells using our unique mouse-rat interspecies chimeric method, we generated a spermatogenesis-impaired mouse using the conditional recombination technique, and were able to generate rat spermary tissue in a mouse with high efficiency. Our project successfully developed an efficient technique to generate gene-modified rats.
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Free Research Field |
分子神経生物学
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