Development of new methods for a gene-manipulated rat for the analysis of human brain function

2015 Fiscal Year Final Research Report

• Project/Area Number: 24240048
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Niigata University
• Principal Investigator: sakimura kenji 新潟大学, 脳研究所, 教授 (40162325)
• Co-Investigator(Kenkyū-buntansha): Abe Manabu 新潟大学, 脳研究所, 准教授 (10334674) ; Natsume Rie 新潟大学, 脳研究所, 技術職員 (60467082) ; Nakatsukasa Ena 新潟大学, 脳研究所, 助教 (60641579) ; Zhou Li 新潟大学, 脳研究所, 特任助教 (80568410)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: ラットES細胞 / 遺伝子組換え / 異種動物間キメラ / ノックアウトラット / 顕微授精

Outline of Final Research Achievements

The aim of this project is to develop an efficient and low-cost method to generate gene-manipulated rats for the analysis of brain functions, so that we can provide the research community of this country with gene-modified rats as useful animal resources. For this purpose, we established an optimum culture condition for rat ES cells, a rapid gene-manipulated method using homologous recombination, and a generation method of chimeras that efficiently differentiate to germ cells. Thus, we succeeded in stable generation of gene-manipulated rats. In addition, in order to produce rat sperm from the rat ES cells using our unique mouse-rat interspecies chimeric method, we generated a spermatogenesis-impaired mouse using the conditional recombination technique, and were able to generate rat spermary tissue in a mouse with high efficiency. Our project successfully developed an efficient technique to generate gene-modified rats.

Free Research Field

分子神経生物学