Establishment of Molecular Genetic Method for Damage-response at Tissue Cell

2014 Fiscal Year Final Research Report

• Project/Area Number: 24241018
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka University
• Principal Investigator: TODO Takeshii 大阪大学, 医学(系)研究科(研究院), 教授 (90163948)
• Co-Investigator(Kenkyū-buntansha): MASATO Kinoshita 京都大学, 大学院農学研究科, 助教 (60263125)
• Co-Investigator(Renkei-kenkyūsha): FUJIWARA Tomoko (ISHIKAWA Tomoko) 大阪大学, 大学院医学系研究科, 助教 (70402922)
• Project Period (FY): 2012-05-31 – 2015-03-31
• Keywords: 損傷応答 / メダカ

Outline of Final Research Achievements

The most important issue for the study of damage-response is an establishment of method for direct analysis of somatic tissue cell. In this study, we have tried to establish novel molecular-genetic method using medaka as experimental model animal. The strategy we have employed is transgenesis-mediated mosaicism by use of efficient gene transfer vector. piggyback (PB) transposon can integrate into genome DNA efficiently when injected into fertilized eggs. We have produced PB-based BAC transfer vector which can transfer large genomic DNA region. Using the PB vector, we have succeeded to establish germ cell transformant and somatic mosaic.

Free Research Field

分子放射線生物学