Induction mechanism of targeted gene expression by controlling spatiotemporally plasma flow at biological interface

2014 Fiscal Year Final Research Report

• Project/Area Number: 24246034
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fluid engineering
• Research Institution: Tohoku University
• Principal Investigator: SATO Takehiko 東北大学, 流体科学研究所, 教授 (10302225)
• Co-Investigator(Kenkyū-buntansha): JOHKURA Kohei 信州大学, 医学部, 准教授 (30303473) ; OHARA Taku 東北大学, 流体科学研究所, 教授 (40211833) ; KANAZAWA Seiji 大分大学, 工学部, 教授 (70224574) ; MIYAHARA Takashi 静岡大学, 工学研究科, 教授 (70239432)
• Co-Investigator(Renkei-kenkyūsha): KOMIYA Atsuki 東北大学, 流体科学研究所, 准教授 (60371142) ; OHTANI Kiyonobu 東北大学, 流体科学研究所, 助教 (80536748) ; YOSHINO Daisuke 東北大学, 流体科学研究所, 助教 (80624816)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオ流体力学 / 自己保存プラズマ流 / 極微小時空間解析 / 細胞応答 / 遺伝子発現

Outline of Final Research Achievements

The development of the formation and controlling method of the self-preserving plasma flow were accomplished by development of the high-speed high-voltage power supply, visualization system which had a very high spatiotemporal resolution and high accurate synchronous measurement system of the plasma characteristics. It has been shown that the self-preserving plasma flow makes a microscopic bubble cluster at the tip of the electrode several hundred nanoseconds after the voltage was applied, then starts propagation intermittently with a speed of about 2 kilometers per second from the tip of the protrusion of the bubbles synchronously with the pulsed discharge current.
As for the induction of gene cluster expression, it has been shown by the comprehensive gene analysis that the gene cluster with functions of metabolism, immunity, regeneration, etc. expressed by weak plasma stimuli, which was different from hydrogen peroxide which was a main cause of the plasma stimuli.

Free Research Field

流体工学