2014 Fiscal Year Final Research Report
Mitochondrial genome editing by TALEN
| Project/Area Number |
24248001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
|
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | TALEN / ミトコンドリアゲノム編集 |
|---|
| Outline of Final Research Achievements |
Although plant mitochondria genome encodes scientifically and/or agriculturally important genes, the detailed analyses are quite limited because the transformation technique of plant mitochondrial genome is still unavailable. This project tried to change the sequence of the targeted genes of Arabidopsis mitochondrial genome by using TALEN, an artificial restriction enzyme. Because each gene-targeting needs a pair of TALEN proteins with mitochondrial localizing signals, many kinds of simultaneously dual expression vectors were made. This laborious step was drastically improved by our developing method with multisite gateway system. T1 plants introduced with these TALEN expression vectors were made and some of their mitochondrial target genes were checked, but at this time point, mutation has not yet been detected.
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| Free Research Field |
植物分子育種学
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