The construction of integrated fungal fermentation process (IFFP)

2014 Fiscal Year Final Research Report

• Project/Area Number: 24248030
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Kyushu University
• Principal Investigator: KONDO Ryuichiro 九州大学, (連合)農学研究科(研究院), 特任教授 (80091370)
• Co-Investigator(Kenkyū-buntansha): HIRAI Hirofumi 静岡大学, 農学部, 教授 (70322138) ; KAMEI Ichiro 宮崎大学, 農学部, 教授 (90526526)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオエタノール / 白色腐朽菌 / 褐色腐朽菌 / リグニン / 微生物分解 / 広葉樹 / 針葉樹 / アルコール発酵

Outline of Final Research Achievements

Future expansion has to be based on bioethanol from lignocellulosic materials, such as agricultural residues and forest residues, as well as from dedicated crops.
The white-rot fungus Phlebia sp. strain MG-60 was proposed as a candidate for integrated fungal fermentation process (IFFP), which unifies aerobic delignification and semi-aerobic consolidated biological processing by a single microorganism based on its ability to efficiently degrade lignin and ferment the sugars from cellulose. To improve IFFP, the development of a molecular breeding method for strain MG-60 is necessary. The study is to establish the transformation method for the strain MG-60 and to obtain the over-expressing transformants of lignin-degrading enzyme, manganese peroxidase.The MG-60 transformation provides a useful methodology for widely accessible to interested researches. These results indicate the possibility of metabolic engineering of strain MG-60 for improving IFFP.

Free Research Field

木質化学