Analyses of molecular mechanisms of gonadal sex differentiation, oocyte growth and maturation for the development of techniques on artificial control of reproduction in sturgeons

2015 Fiscal Year Final Research Report

• Project/Area Number: 24248033
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: Adachi Shinji 北海道大学, 水産科学研究科(研究院), 教授 (40231930)
• Co-Investigator(Kenkyū-buntansha): ARAI Katsutoshi 北海道大学, 大学院水産科学研究院, 教授 (00137902) ; TAKAGI Yasuaki 北海道大学, 大学院水産科学研究院, 教授 (10212002) ; HIRAMATSU Naoshi 北海道大学, 大学院水産科学研究院, 准教授 (10443920) ; YAMAHA Etsuro 北海道大学, 北方生物圏フィールド科学センター, 教授 (60191376) ; IJIRI Shigeho 北海道大学, 大学院水産科学研究院, 准教授 (90425421)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: チョウザメ / 性分化 / 卵成長 / 卵成熟能 / 排卵能 / 生殖腺刺激ホルモン / 次世代シーケンサー / 性統御技術

Outline of Final Research Achievements

Identification of genes that express by stage specific manner and quantification of their mRNA levels in gonads were conducted throughout the periods of molecular sex differentiation, morphological sex differentiation, oocyte growth and maturation in Russian and Amur sturgeons using a next-generation sequencer. The information obtained from this study was utilized in the application for the development of techniques on sexing in the early developmental stage, induction of sexual maturation in the earlier gonadal developmental stage, production of good quality eggs and production of all female population.

Free Research Field

魚類生殖生理学