化学物質汚染に対する産業動物の生体防御機構の研究基盤

2015 Fiscal Year Annual Research Report

• Project/Area Number: 24248056
• Research Institution: Hokkaido University
• Principal Investigator: 石塚 真由美 北海道大学, (連合)獣医学研究科, 教授 (50332474)
• Co-Investigator(Kenkyū-buntansha): 池中 良徳 北海道大学, (連合)獣医学研究科, 准教授 (40543509)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 産業動物 / 環境汚染 / シトクロムP450 / グルクロン酸抱合酵素 / 獣医学

Outline of Annual Research Achievements

産業動物の化学物質汚染は食の安全に直結する重要な問題でもあるが、化学物質の感受性の種差や個体差に関する研究は極めて少なく、これがケミカルハザードを引き起こす大きな原因の一つとなっている。本研究では、産業動物が有する化学物質に対する生体防御能力について、以下を明らかにした。なお、試料については予定通り、国内およびアフリカ諸国からのサンプルを分析に用いた。多様な動物種を補完するために、一部タイなどアジア地域からの試料についても解析を行った。
１）反芻類を中心として、産業動物に蓄積する環境化学物質の解析した。特に、残留性有機汚染物質、多感芳香族類、農薬、重金属類について、反芻獣を中心蓄積する汚染物質の分析を行い、どのような化学物質に暴露されているのか明らかにした。また、蓄積部位の特徴について解析した。反芻中の尿からは農薬や多環芳香族類が検出され、日常的にこれらの化学物質に暴露されていることが明らかとなった。また、舌など可食部位にも鉛をはじめとする金属類が蓄積していた。
２）産業動物として哺乳類と、ニワトリやウズラ、ダチョウなど家禽類における化学物質の代謝酵素類の発現と化学物質の代謝の特徴について明らかにした。哺乳類では、ブタ、ウシ、ウマなどについて、ピレンをモデル化化合物としてその代謝能を分析した。鳥類では、ピレンのほか、多数の水酸化部位を持つワルファリンをモデル化合物として、その代謝能を明らかにした。また、ニワトリおよびダチョウについて、発現するシトクロムP450や第II相抱合酵素を網羅的に解析し、鳥類と哺乳類の種差について明らかにした。
３）反芻獣に多く蓄積するカロテノイドが薬物代謝酵素の発現や活性に及ぼす影響について調べた。カロテノイドが汚染物質による薬物代謝酵素群の発現誘導を一部抑制することを明らかにした。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] ノースウェスト大学/ヨハネスブルグ大学(南アフリカ)
  • Country Name: SOUTH AFRICA
  • Counterpart Institution: ノースウェスト大学/ヨハネスブルグ大学
• [Int'l Joint Research] クワメエンクルマ大学(ガーナ)
  • Country Name: GHANA
  • Counterpart Institution: クワメエンクルマ大学
• [Int'l Joint Research] ザガジック大学(エジプト)
  • Country Name: EGYPT
  • Counterpart Institution: ザガジック大学
• [Int'l Joint Research] カセサート大学(タイ)
  • Country Name: THAILAND
  • Counterpart Institution: カセサート大学
• [Int'l Joint Research] ザンビア大学(ザンビア)
  • Country Name: ZAMBIA
  • Counterpart Institution: ザンビア大学
• [Int'l Joint Research]
  • # of Other Countries: 1
• [Journal Article] Distribution and health risk assessment of organochlorine pesticides (OCP) residue in edible cattle tissues from northeastern part of Egypt: High accumulation level of OCP in tongue. (2016)
  • Author(s): Mahmoud AFA, IkenakaY, YohannesYB, DarwishWS, Eldaly EA, Morshdy AE, Nakayama SMM, MizukawaH, Ishizuka M.
  • Journal Title: Chemosphere Volume: 144 Pages: 1365-1371
  • DOI: 10.1016/j.chemosphere.2015.10.016.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reliability of stable Pb isotopes to identify Pb sources and verifying biological fractionation of Pb isotopes in goats and chickens. (2016)
  • Author(s): Nakata H, Nakayama SMM, Yabe J, Liazambi A, Mizukawa H, Darwish WS, Ikenaka Y, Ishizuka M
  • Journal Title: Environ Pollut. Volume: 208 Pages: 395-403
  • DOI: 10.1016/j.envpol.2015.10.006.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Partial cloning of CYP2C23a genes and hepatic protein expression in eight representative avian species. (2015)
  • Author(s): Watanabe KP, Kawai YK, Nakayama SMM, Ikenaka Y, Mizukawa H, Takaesu N, Ito M, Ikushiro S, Sakaki T, Ishizuka M.
  • Journal Title: Journal of Veterinary Pharmacology and Therapeutics Volume: 38 Pages: 190-195
  • DOI: 10.1111/jvp.12159
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Human health risks from metals and metalloid via consumption of food animals near Gold Mines in Tarkwa, Ghana: Estimation of the daily intakes and target hazard quotients (THQs). (2015)
  • Author(s): Bortey-Sam N, Nakayama SMM, Ikenaka Y, Akoto O, Yohannes YB, Baidoo E, Mizukawa H, Ishizuka M.
  • Journal Title: Ecotoxicology and Environmental Safety Volume: 111 Pages: 160-167
  • DOI: 10.1016/j.ecoenv.2014.09.008
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Lead poisoning in children from townships in the vicinity of a lead-zinc mine in Kabwe, Zambia. (2015)
  • Author(s): Yabe J, Nakayama SMM, Ikenaka Y, Yohannes YB, Bortey-Sam N, Oroszlany B, Muzandu K, Choongo K, Kabalo AN, Ntapisha J, Mweene A, Umemura T, Ishizuka M
  • Journal Title: Chemosphere Volume: 119 Pages: 941-947
  • DOI: 10.1016/j.chemosphere.2014.09.028
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Levels, potential sources and human health risk of polycyclic aromatic hydrocarbons (PAHs) in particulate matter (PM10) in Kumasi-Ghana. (2015)
  • Author(s): Bortey-Sam N, Ikenaka Y, Akoto O, Nakayama M.M.S, Yared YB, Baidoo E, Mizukawa H, Ishizuka M.
  • Journal Title: Environ Sci Pollut Res Volume: 22 Pages: 9658-67
  • DOI: 10.1007/s11356-014-4022-1
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Are red gourami (Colisa labiosa) low xenobiotic metabolizers? Elucidation of in vivo pharmacokinetics of pyrene as a model substrate. (2015)
  • Author(s): Ikenaka Y, Nakayama SMM, Oguri M, Saengtienchai A, Mizukawa H, Kobayashi J, Darwish WS, Ishizuka M.
  • Journal Title: Environ Toxicol Pharmacol, Volume: 39 Pages: 1148-1153
  • DOI: 10.1016/j.etap.2015.04.004.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization and tissue distribution of conjugated metabolites of pyrene in the rat. (2015)
  • Author(s): Saengtienchai A, Ikenaka Y, Darwish WS, Nakayama SMM, Mizukawa H, Ishizuka M.
  • Journal Title: J Vet Med Sci. Volume: 77 Pages: 1261-1267
  • DOI: 10.1292/jvms.14-0632.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metal extent in blood of livestock from Dandora dumping site, Kenya: source identification of Pb exposure by stable isotope analysis. (2015)
  • Author(s): Nakata H, Nakayama SMM, Ikenaka Y, Mizukawa H, Ishii C, Yohannes YB, Konnai S, Darwish WS, Ishizuka M
  • Journal Title: Environ Pollut. Volume: 205 Pages: 8-15
  • DOI: 10.1016/j.envpol.2015.05.003.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Incidence and public health risk assessment of toxic metal residues (cadmium and lead) in Egyptian cattle and sheep meats. (2015)
  • Author(s): Darwish WS, Hussein MA, El-Desoky KI, Ikenaka Y, Nakayama SMM, Mizukawa H, Ishizuka M.
  • Journal Title: Int Food Res J, Volume: 22 Pages: 1719-1726
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metabolic Activation of Heterocyclic Amines and Expression of Xenobiotic-Metabolizing Enzymes in the Gastrointestinal Tract of Rats. (2015)
  • Author(s): Darwish WS, Nakayama SMM, Itotani Y, Ohno M, Ikenaka Y, Ishizuka M.
  • Journal Title: J Food Sci, Volume: 80 Pages: T1627-T1632
  • DOI: 10.1111/1750-3841.12931.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Health risk assessment of heavy metals and metalloid in drinking water from communities near gold mines in Tarkwa, Ghana. (2015)
  • Author(s): Bortey-Sam N, Nakayama SMM, Ikenaka Y, Akoto O, Baidoo E, Mizukawa H, Ishizuka M
  • Journal Title: Environ Monit Assess, Volume: 187 Pages: 1-12
  • DOI: 10.1007/s10661-015-4630-3.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Uridine diphosphate-glucuronosyltransferase (UGT) xenobiotic metabolizing activity and genetic evolution in Pinniped species. (2015)
  • Author(s): 9.Kakehi M, Ikenaka Y, Nakayama SMM, Kawai YK, Watanabe KP, Mizukawa H, Nomiyama K, Tanabe S, Ishizuka M.
  • Journal Title: Tox Sci. Volume: 147 Pages: 360-369
  • DOI: 10.1093/toxsci/kfv144.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytochrome P450-mediated warfarin metabolic ability is not a critical determinant of warfarin sensitivity in avian species: In vitro assays in several birds and in vivo assays in chicken. (2015)
  • Author(s): Watanabe KP, Kawata M, Ikenaka Y, Nakayama SMM, Ishii C, Darwish WS, Saengtienchai A, Mizukawa H, Ishizuka M.
  • Journal Title: Environ Toxicol Chem. Volume: 34 Pages: 2328-2334
  • DOI: 10.1002/etc.3062.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Accumulation of heavy metals and metalloid from agricultural soils to foodstuffs around Tarkwa area in Ghana, and associated human health risks. (2015)
  • Author(s): Bortey-Sam N, Nakayama SMM, Akoto O, Ikenaka Y, Fobil N.J., Baidoo E, Mizukawa H, Ishizuka M.
  • Journal Title: Int J Environ Res Public Health. Volume: 12 Pages: 8811-8827
  • DOI: 10.3390/ijerph120808811.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ecological Risk of Heavy Metals and a Metalloid in Agricultural Soils in Tarkwa, Ghana. (2015)
  • Author(s): Bortey-Sam N, Nakayama SMM, Akoto O, Ikenaka Y, Fobil N.J., Baidoo E, Mizukawa H, Ishizuka M.
  • Journal Title: Int J Environ Res Public Health. Volume: 12 Pages: 11448-11465
  • DOI: 10.3390/ijerph120911448.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] β-carotene and retinol contents in the meat of herbivorous ungulates with a special reference to their public health importance. (2015)
  • Author(s): Darwish WS, Ikenaka Y, Morshdy AE, Eldesoky KI, Nakayama SMM, MizukawaH, Ishizuka M.
  • Journal Title: JVMS Volume: 78 Pages: 351-354
  • DOI: 10.1292/jvms.15-0287.
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 非実験動物における化学物質代謝能の特徴と種差 (2016)
  • Author(s): 池中良徳、筧麻友、水川葉月、中山翔太、石塚真由美
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 2016-03-26 – 2016-03-29
  • Invited
• [Presentation] Metabolism and biotransformation of organohalogen compounds in the liver microsomes of cats and dogs. (2015)
  • Author(s): Hazuki Mizukawa, Kei Nomiyama, Nozomu, Yokoyama, Osamu Ichii, Mitsuyoshi Takiguchi, Yoshinori Ikenaka, Shouta M.M. Nakayama,Misaki Maehara, Shinsuke Tanabe, Mayumi Ishizuka.
  • Organizer: SETAC North America 36th Annual Meeting,
  • Place of Presentation: Salt Palace Convention Center, Salt Lake City, USA
  • Year and Date: 2015-11-01 – 2015-11-05
  • Int'l Joint Research
• [Presentation] 鳥類で起こっているケミカルハザードとそのメカニズム (2015)
  • Author(s): 中山翔太、水川葉月、池中良徳、石塚真由美
  • Organizer: 第21回日本野生動物医学会大会
  • Place of Presentation: 酪農学園大学、北海道
  • Year and Date: 2015-07-30 – 2015-08-02
  • Invited
• [Presentation] Vector-control pesticides disseminating via international waterways to accumulate in free-ranging freshwater and marine fish species (2015)
  • Author(s): Lesa A Thompson, Yoshinori Ikenaka, Johannes J van Vuren, Victor, Wepener, Nico Smit, Wynand Vlok, Yared B. Yohannes, Hazuki Mizukawa, Shouta M.M. Nakayama, Mayumi Ishizuka.
  • Organizer: Vth International Wildlife Management Congress
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2015-07-26 – 2015-07-30
  • Int'l Joint Research
• [Presentation] Lead poisoning and source identification in raptors from Japan. (2015)
  • Author(s): Chihiro Ishii, Shouta M.M. Nakayama, Yoshinori Ikenaka, Hazuki Mizukawa, Yared B. Yohannes, Hokuto Nakata, Keisuke Saito, Yukiko Watanabe, Shinsuke Tanabe, Kei Nomiyama, Terutake Hayashi, Yasushi Masuda, Kentaro Q. Sakamoto, Mayumi Ishizuka
  • Organizer: Vth International Wildlife Management Congress
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2015-07-26 – 2015-07-30
  • Int'l Joint Research
• [Remarks] 北海道大学大学院獣医学研究科毒性学教室
  • URL: http://tox.vetmed.hokudai.ac.jp/