Investigation of optimal nutritional balance based on the inhibition of S-(2-succinyl) cysteine (2SC), post-translational modification by fumarate

2014 Fiscal Year Final Research Report

• Project/Area Number: 24300260
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Tokai University
• Principal Investigator: NAGAI Ryoji 東海大学, 農学部, 准教授 (20315295)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Yukio 熊本大学, 生命科学研究科, 講師 (70452886) ; ARAKI Tomohiro 東海大学, 農学部, 教授 (20193071)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 翻訳後修飾 / 脂肪細胞 / 生活習慣病 / フマル酸 / 糖尿病

Outline of Final Research Achievements

We previously demonstrated that S-(2-succinyl)cysteine(2SC) is generated by the reaction between cysteine and fumarate in adipocytes under hyperglycemic conditions. In the present study, we established the measurement system of 2SC in order to evaluate the mitochondrial disorders without tissue destruction. As results, 2SC was detected not only in serum but also in solid tissues such as hair and nail. Especially 2SC was detectable with 5 microL serum that was obtained from the blood collection from the fingertip. Serum 2SC was increased by the pathogenesis of kidney failures, patients in hypercholesteremia, and people who do not eat vegetables.

Free Research Field

生化学　食品化学　分析化学