Biochemical study on the molecular mechanisms of induced mutagenesis and post-replication repair pathways

2015 Fiscal Year Final Research Report

• Project/Area Number: 24310040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Nagoya University
• Principal Investigator: Masuda Yuji 名古屋大学, 医学系研究科(環医), 准教授 (30273866)
• Co-Investigator(Renkei-kenkyūsha): MASUTANI Chikahide 名古屋大学, 環境医学研究所, 教授 (40241252) ; Kanao Rie 名古屋大学, 環境医学研究所, 助教 (30542287)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 複製後修復

Outline of Final Research Achievements

Radiation and environmental mutagens induce mutations. To elucidate molecular mechanisms of the induced mutagenesis is one of the biggest issues in this field. A large fraction of the induced mutation is generated by a cellular process, post-replication repair pathway. In humans, cells have two sub-pathways. One is the error-prone pathway, translesion DNA synthesis (TLS). The other is the error-free, in principle, pathway, template switch (TS). The regulation of the pathway choice that is a crucial step for the maintenance of genetic stability is regulated by ubiquitination of PCNA. In this study, we established in vitro reconstitution systems for PCNA ubiquitination and deubiquitination, and analyzed molecular mechanisms of these biochemical reactions.

Free Research Field

生化学