2014 Fiscal Year Final Research Report
Split molecular catalyst and its analytical application as a signal amplifier
| Project/Area Number |
24350040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Toshihiko 鳥取大学, 工学部, 准教授 (10332868)
IMAHORI Tatsushi 東京理科大学, 工学部, 講師 (90433515)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | DNA / DNAコンジュゲート / 機能性核酸 / アプタマー / DNAzyme / 金属イオン / シクロデキストリン |
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| Outline of Final Research Achievements |
Chemically engineered DNAs, global conformation of which can be modulated in response to specific stimuli, could be allosteric functional DNAs or work as a modulator of the functional nucleic acids such as DNAzymes and aptamers. We showed that two terpyridines built in the DNA backbone form a stable intramolecular 1 : 2 complex, [M(terpy)2]2+, with divalent transition metal ions. Upon complexation, the DNA conjugates adopt a Ω-shape structure in which two distal sequences located outside the terpyridines connect with each other to form a continuous segment with a specific structure or sequence. Such DNA structure is globally controlled by local metal complexation events that can be rationally designed based on general coordination chemistry. This method is regarded as metal ion-directed dynamic sequence edition or DNA splicing. Split DNAzymes with peroxidase-like activity can thus be regulated by several transition metal ions through sequence edition techniques based on the Ω-motif.
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| Free Research Field |
核酸化学、生物分析化学
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