Quantitative analysis of DNA on GO nanosurface and development of DNA binding ligand screening system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24350089
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Konan University
• Principal Investigator: MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 教授 (50388758)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: グラフェン / DNA / 熱力学的解析 / 速度論的解析 / テロメア / 四重らせん構造 / リガンド / バイオセンサー

Outline of Final Research Achievements

Graphene oxide (GO) is widely used in biosensors, because GO can absorb a fluorophore-labeled DNA and efficiently quench the fluorescence. However, the adsorption process remains unclear yet.
In this study, we have studied the following topics. (1) Properties of various DNA structures in non-ideal conditions, as observed in the GO nanosurface: It was found that hydration state of DNA strands was critically important for structure and thermodynamic stability of DNAs under non-ideal conditions. (2) The adsorption mechanism of DNA onto GO nanosurface: We studied thermodynamically and kinetically the mechanism, and found that the binding affinity of DNA was independent of the DNA structure but kinetics was largely depending on the structure. (3) Development of sensing ligands and screening systems for DNA G-quadruplexes: We found ThT, a typical fluorescence probe for protein fibril, was able to bind specifically to DNA G-quadruplex with bright fluorescence signals.

Free Research Field

分子設計化学