Development of technologies for maintaining undifferentiated state and inducing growth and differentiation of human iPS cell by using chimeric receptor

2014 Fiscal Year Final Research Report

• Project/Area Number: 24360337
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: The University of Tokyo
• Principal Investigator: NAGAMUNE Teruyuki 東京大学, 工学(系)研究科(研究院), 教授 (20124373)
• Co-Investigator(Renkei-kenkyūsha): KAWAHARA Masahiro 東京大学, 大学院工学系研究科, 講師 (50345097) ; YAMAGUCHI Satoshi 東京大学, 先端科学技術研究センター, 講師 (80398106)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: サイトカイン受容体 / キメラ蛋白質 / シグナル伝達 / 未分化維持技術 / 増殖・分化技術

Outline of Final Research Achievements

To realize regenerative medicine, a technology to efficiently prepare a large number of differentiated cells of interest at low cost is necessary. However, differentiation-inducing factors such as cytokines are generally expensive. In this study, we designed chimeric receptors, in which the extracellular domain of cytokine receptors is replaced by single-chain antibody, which enables signaling in response to a cheap antigen instead of expensive cytokines. We aimed to develop a system to keep and expand stem cells in an undifferentiated state, and to differentiate them into specific lineages by expressing the cheap antigen-responsive chimeric receptors on the cell surface.

Free Research Field

細胞工学、蛋白質工学