Transcriptome sequencing in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica) and seasonal profiling of the expressed genes throughout a growing season

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380098
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: FUJIWARA Takeshi 独立行政法人森林総合研究所, 木材特性研究領域, 室長 (00353839)
• Co-Investigator(Kenkyū-buntansha): WATANABE Atsushi 九州大学, 農学研究院, 准教授 (10360471) ; NOSE Mine 森林総合研究所, 林木育種センター, 研究員 (20582753) ; KURITA Manabu 森林総合研究所, 林木育種センター, 室長 (40370829) ; IKI Taiichi 森林総合研究所, 林木育種センター, 主任研究員 (40370845) ; ORIBE Yuichiro 森林総合研究所, 東北育種場, 課長 (40370853) ; YAMASHITA Kana 森林総合研究所, 木材特性研究領域, 主任研究員 (60353900) ; KURODA Katsushi 森林総合研究所, 木材特性研究領域, 主任研究員 (90399379)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: スギ / EST / マイクロアレイ / 木部形成

Outline of Final Research Achievements

Japanese cedar is one of the most valuable species for wood industries and it has been target species of tree breeding program because of its wide diversity in wood properties. Thus, molecular aspects of wood formation offer practical information that can be used for screening and forward genetic approaches to improve wood quality.
Total RNA was isolated from samples including cambium and differentiating xylem collected from trunks of plus trees and 55,051 unique sequences were obtained. After identifying expressed sequence tags, we designed a custom cDNA microarray with 18,082 probes to compare expression of highly regulated genes throughout a growing season. Among various types of the gene expression profiles provided by the microarray, groups of genes regulated in growing season were detected, which were assigned to the functional classes such as “Carbohydrate transport and metabolism” and “Cell wall/membrane/envelope biogenesis” which are supposed to relate with wood formation.

Free Research Field

木材組織学