Analyses of genes involved in cell polarity in tissues and animals in situ

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: HARADA Akihiro 大阪大学, 医学(系)研究科(研究院), 教授 (40251441)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Shin'ichiro 大阪大学, 大学院医学系研究科, 助教 (60584521) ; KUNII Masataka 大阪大学, 大学院医学系研究科, 助教 (80614768)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞極性 / 極性輸送 / ノックアウトマウス / apical

Outline of Final Research Achievements

We published papers containing analyses of Rab8a,b, PKD1,2, and FAPP2 knockout mice. We finished analysis of pancreas-specific SNAP23 knockout mice and identified molecular mechanism for increased insulin secretion in these mice. The results were summarized and submitted to a journal. We are analyzing the molecular mechanisms underlining phenotypes of syntaxin3 knockout mice by generating KO cell lines by CRISPR/Cas9 method. For identifying molecular mechanism of apical transport, we discovered a Rab8a binding protein and its role for apical transport. For monitoring the transport of apical proteins, we generated a model protein and introduced it into cultured intestinal organoids. We found out novel candidate molecules for apical transport using C. elegans. We generated knockout mice lacking these molecules and analyzing their phenotypes.

Free Research Field

細胞生物学