Quality control of insulin granule exocytosis

2015 Fiscal Year Final Research Report

• Project/Area Number: 24390068
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Gunma University
• Principal Investigator: IZUMI Tetsuro 群馬大学, 生体調節研究所, 教授 (00212952)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: インスリン / 分泌顆粒 / 開口放出 / 細胞内膜輸送 / 糖尿病

Outline of Final Research Achievements

We have analyzed the function of multiple Rab27 effector proteins expressed in pancreatic beta cells. As a result, we have identified novel roles of three Rab27 effector proteins in insulin granule exocytosis. First, Noc2 has a role in connecting maturation and exocytosis steps by interacting with both Rab2a and Rab27a. Second, exophilin8 is involved in the transport of granules to cell periphery by binding a novel protein. Third, granuphilin is an exclusive component of the functional and fusion-inhibitory docking machinery of secretory granules. Thus, three kinds of Rab27 effector proteins function in completely different exocytic processes in pancreatic beta cells.

Free Research Field

分子細胞生物学