2015 Fiscal Year Final Research Report
Molecular mechanisms for signals generated by the interaction between glycosphingolipids on the cell membrane and their ligands
| Project/Area Number |
24390078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Chubu University (2015)
Nagoya University (2012-2014) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 糖脂質 / 細胞膜 / ミクロドメイン / シグナル / リガンド / 癌関連抗原 |
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| Outline of Final Research Achievements |
We have analyzed cell signaling generated by the interaction between glycosphingolipids expressed on cancer cells and neighboring membrane molecules expressed in the vicinity of them, and aimed to apply the results in the construction of cancer therapeutic strategies targeting membrane microdomain leading to obtain fundamental information by clarifying the implication of the signals in the regulation of cell phenotypes.
In order to do so, we used Enzyme-mediated activation of radical sources (EMARS)/mass spectrometry, to identify the interacting molecules with cancer-associated glycolipids. In melanomas, we identified neogenin as a GD3-associated molecule, in human gliomas, EphA2 as a GD2 associated molecule, and in small cell lung cancers, ESCT2 was identified as a GD2-associated molecule. Furthermore, PDGF receptor alpha was also identified as GD3-associated molecule in murine glioma cells. Then, individual molecular functions of these membrane molecules have been investigated.
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| Free Research Field |
生化学、糖鎖生物学
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