Analysis of mechanisms by which proteostasis capacity is regulated through activation of heat shock factor

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Yamaguchi University
• Principal Investigator: NAKAI Akira 山口大学, 医学(系)研究科(研究院), 教授 (60252516)
• Co-Investigator(Kenkyū-buntansha): FUJIMOTO Mitsuaki 山口大学, 大学院医学系研究科, 准教授 (80359900) ; HAYASHIDA Naoki 山口大学, 大学院医学系研究科, 講師 (40420517) ; TAKII Ryosuke 山口大学, 大学院医学系研究科, 助教 (00419558)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 神経変性疾患 / 熱ショック / 転写 / クロマチン / プロテオスタシス / リン酸化

Outline of Final Research Achievements

Capacity of cellular protein homeostasis, known as proteostasis, is related with progression of age-related neurodegenerative disease and cancer. One of prominent mechanisms of the maintenance of proteostasis capacity is the heat shock response, which is regulated mainly at the level of transcription by heat shock factors (HSFs). Among mammalian HSFs, HSF4 constitutively binds to DNA under unstressed conditions. In this research project, we demonstrate a mechanism by which HSF4 activity is regulated through phosphorylation, and suggest that proteostasis capacity is regulated by a chemical modification of HSF4 under physiological conditions.

Free Research Field

医歯薬学、基礎医学、病態医化学、熱ショック応答と分子シャペロン