Phosphoproteomic analysis of cardiac proteins modulated by hypothermia and rewarming

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390328
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Shimane University
• Principal Investigator: ODA Teiji 島根大学, 医学部, 教授 (50448198)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Kenichi 島根大学, 総合科学研究支援センター, 教授 (30202328) ; SHIMIZU Koji 島根大学, 医学部, 助教 (70548578) ; SUEHIRO Shoichi 島根大学, 医学部, 助教 (90596545)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 低体温 / 心筋 / 蛋白質 / リン酸化 / 心機能 / 自律神経 / プロテオミクス

Outline of Final Research Achievements

Hypothermia is utilized in cardiac/aortic surgery to protect organs from ischemic injury. Because of a short duration of hypothermia, it is important as for a principal mechanism of hypothermia to modulate protein phosphorylation rather than de novo protein synthesis. To analyze hypothermia-induced protein phosphorylation in rat heart muscle, we employed nanoLC-MALDI/TOF/TOF MS/MS tandem mass spectrometry. In total, 317 phosphopeptides were identified, in which 27 peptides were quantitatively identified with significant evidences. These were included in 11 proteins that regulate ATP synthesis, muscle contraction and protein folding. Mild (30℃) to deep hypothermia (23℃) augmented contractility of left ventricle; which were associated with phosphorylation of the cardiac myofilament proteins including myosin-6 and tropomyosin. Hypothermic modulation of protein phosphorylation could cause the hypothermic protection from ischemic injury, which needs to be investigated further.

Free Research Field

心臓血管外科学