2015 Fiscal Year Final Research Report
Simple Domestic Gene Therapy against Anti-cancer Angiogenesis with Decoy Oligonucleotides.
| Project/Area Number |
24390420
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kanazawa Medical University (2014-2015)
Shimane University (2012-2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ONIMARU Mitsuho 九州大学, 医学研究院, 助教 (00380626)
NAKAYAMA Eiji 北海道医療大学, 歯学部, 教授 (60172467)
SUGIMOTO Naotoshi 金沢大学, 医学系, 准教授 (80272954)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 悪性新生物 / 遺伝子治療 / 血管新生 |
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| Outline of Final Research Achievements |
Among angiogenic facotrs produced by tumor cells, vascular endothelial growth factor (VEGF) seems to be the most potent and pathophysiologically important, and its synthesis has been shown to be modulated through the activation of some transcription factors including HIF-1 function under hypoxic condition. We transferred HIF-1 decoy ODNs, composed of synthetic double-stranded DNA including consensus sequence of binding site of the HIF-1, into cultured cancer cells (SAS cells) by the HVJ-liposome method. The overexpression of VEGF by cancer cells stimulated by hypoxia could be apparently suppressed by the transfection of HIF-1 decoy ODNs, but not by mt-HIF-1 decoy ODNs. These results suggested that the HIF-1 decoy strategy would be effective for regulating tumor growth by reducing angiogenic acitivity of cancer cells through HIF-1-mediated gene transactivations.
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| Free Research Field |
実験腫瘍学
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