Development of a novel method using macrophage-activated for therapy against chronic muscle pain

2015 Fiscal Year Final Research Report

• Project/Area Number: 24390429
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Tohoku University
• Principal Investigator: TSUCHIYA MASAHIRO 東北大学, 歯学研究科(研究院), 大学院非常勤講師 (60372322)
• Co-Investigator(Kenkyū-buntansha): SASAKI Keiichi 東北大学, 大学院歯学研究科, 教授 (30178644) ; HAGIWARA Yoshihiro 東北大学, 大学院医学研究科, 准教授 (90436139) ; KUROISHI Yoshinobu 東北大学, 大学院歯学研究科, 助教 (30400261)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 筋疲労 / 顎関節症 / IL-1 / マクロファージ / 糖代謝

Outline of Final Research Achievements

Pathological mechanism of chronic muscle pain associated with temporomandibular joint disease (TMD) is still unclear. Using a unique masticatory model, we examined whether macrophages (Mφ) plays roles to recover from muscle fatigue. The masticatory activity didn't increase the number of Mφ in masseter muscle. On the other hand, Mφ depletion by the administration of clodronate-liposome (i.v.) significantly decrease the masticatory-like behavior. In addition, the decrease of IL-1β expression level in the masseter followed by the impairment of IL-6 induction have been observed. Interestingly, we also confirmed that the failure of glucose uptake in skeletal muscle tissues. Our data also underscore the importance of Mφ in IL-1-mediated local interplay between autocrine myokines including IL-6 and paracrine cytokines in active skeletal muscles. This interplay is directly involved in MM performance and fatigability, perhaps mediated through maintaining muscular glucose homeostasis.

Free Research Field

歯科補綴学