Elucidation of cytokine network in oral squamous cell carcinoma stem cell niche, and its application to diagnosis/treatment

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390456
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: OKAMOTO TETSUJI 広島大学, 医歯薬保健学研究院, 教授 (00169153)
• Co-Investigator(Kenkyū-buntansha): HAYASHIDO Yasutaka 広島大学, 病院, 講師 (70243251)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 口腔扁平上皮癌細胞 / 癌幹細胞 / CD133 / CD44 / side population / rBC2LCN / 無血清培養 / sphere形成

Outline of Final Research Achievements

In this study, we aimed to establish a new diagnosis and treatment that target cancer stem cells along with the elucidation of the carcinogenesis of oral squamous cell carcinoma (OSCC). For that, the cancer stem cells of OSCC, will be separated, and identified in serum-free culture. Then the niche mechanism of OSCC will be clarified. We examined the usefulness of CD133(+), Sp and rBC2LCN that is specifically expressed in ES / iPSCs, as a stem cell marker of OSCC.
As a result, positive cell ratio of CD133 (+), rBC2LCN (+), and Sp is 0.5%, 2%, and 1%, respectively. These cells exhibited a high sphere forming ability in suspension culture. EGF and sonic hedgehog induces sphere forming ability. Sp showed resistance to anti-cancer drug and high tumorigenicity. rBC2LCN I was considered to be useful as a novel OSCC stem cell marker.

Free Research Field

口腔外科学