2014 Fiscal Year Final Research Report
Development of a novel technique for analyzing anatomical and functional difference in the nigral dopamine neurons by the use of viral vectors.
| Project/Area Number |
24500379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MASAHIKO Takada 京都大学, 霊長類研究所, 教授 (00236233)
MATSUMOTO Masayuki 筑波大学, 医学医療系, 教授 (50577864)
INOUE Satoshi 国立感染症研究所, 獣医科学部, 室長 (90213157)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 神経科学 / 脳・神経 / バイオテクノロジー / ウイルスベクター / 解剖学 |
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| Outline of Final Research Achievements |
In this study, we developed a novel experimental technique that enabled us to analyze anatomical and functional difference in the nigral dopamine neurons. First, we improved a pathway-selective expression system we recently developed to increase gene expression and decrease leak expression. Second, we refined a rabies virus vector we recently established to increase foreign gene expression, and created G-deleted vector. These improvement enable us to visualize the input-output pattern of nigral dopamine neurons. Moreover, using a viral vector expressing channelrhodopsin-2, we demonstrated that selective stimulation of the pathway from the frontal eye field to the superior colliculus affected neuronal activity. These results indicate that the viral vector systems created in this study can be used for analyzing anatomical and functional difference in the specific neuron groups such as nigral dopamine neurons.
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| Free Research Field |
神経科学、ウイルス学
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