Applied research of multi-biosensor using light addressable potentiometric sensor

2015 Fiscal Year Final Research Report

• Project/Area Number: 24500519
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Waseda University
• Principal Investigator: Kuroiwa Shigeki 早稲田大学, ナノ・ライフ創新研究機構, 主任研究員 (90313212)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: バイオイメージング / 分子間相互作用 / バイオセンサ

Outline of Final Research Achievements

A light addressable potentiometric sensor (LAPS) is a method to detect a change of surface potential resulted from the adsorption of molecules as the change of the amplitude of photocurrent. This study was to extend the application of LAPS for medical diagnoses as a molecular interaction imaging device and a borderless multi-biosensor. I established the method to copy patterns of the protein on the LAPS sensor reproducibly to prove the usefulness of LAPS. With regard to the most important technique of surface modification of the sensor, I developed the methods of chemical modification and addition of surfactant to reinforce the electric charge of target molecules, because a LAPS detects the intrinsic electric charge of target molecules.

Free Research Field

ナノバイオサイエンス、生物物理学