2014 Fiscal Year Final Research Report
A novel pathogenic and defence mechanisms in diabetic angiopathy
| Project/Area Number |
24500967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Ibaraki Christian University (2013-2014)
Yamagata University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATO Hideyo 新潟大学, 医学部・保健学科, 教授 (60235380)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 糖尿病性血管障害 / 酸化ストレス / 血管内皮細胞 / 培養細胞 / グルタチオン / 糖尿病 / 血管障害 |
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| Outline of Final Research Achievements |
To investigate the novel mechanism involved in oxidative stress-mediated diabetic angiopathy, we focused on function of the glutathione (GSH) redox cycle in endothelial cells. In diabetes model human umbilical vein endothelial cells (HUVEC) of the living state, the H2O2 scavenging activity via the GSH cycle was significantly reduced. We have carried out the synthesis of the causative agent. Although the synthetic compound was initially unstable, it was possible to obtain a final stable compound at a high purity. We found that this compound inhibits the H2O2 scavenging enzyme. As a protective factor of diabetic angiopathy, we found that myricetin, a food ingredient, inhibits apoptosis in diabetes model HUVEC. These substances may, at least in part, be directly involved in a novel mechanism leading from hyperglycemia to develop vascular injury.
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| Free Research Field |
生化学、基礎栄養学、栄養機能学、病態生化学
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