Characteristics of High-Risk HPV-Associated Pharyngeal Cancers

2014 Fiscal Year Final Research Report

• Project/Area Number: 24501346
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: FURUTA Reiko 公益財団法人がん研究会, その他部局等, 研究員 (10260077)
• Co-Investigator(Kenkyū-buntansha): KITAGAWA Tomoyuki 公益財団法人がん研究会がん研究所, 名誉所長 (50085619) ; YAMAMOTO Noriko 公益財団法人がん研究会がん研究所, 病理部, 主任研究員 (10280629) ; KAWABA Kazuyoshi 公益財団法人がん研究会有明病院, 頭頸科, 部長 (10204760) ; TAKESHIMA Nobuhiro 公益財団法人がん研究会有明病院, 婦人科, 部長 (70216886)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: HPV / 咽頭がん / 異常核分裂像 / ECAC

Outline of Final Research Achievements

Detection of HPV in oropharyngeal squamous cell carcinomas (OCs) is important because HPV-positive OCs are more sensitive to chemo-radiotherapy. We analyzed the HPV status in 119 OC cases (F:M=18:101), using formalin-fixed, paraffin-embedded materials, and 13 type-specific probes of Clinichip HPV (Sekisui), nested PCR and in situ hybridization.
HPV DNA was detected in 74 of 119 cases (62%), F:M=15:59, while HPV was negative in 45 of 119 cases (38%), F:M=3:42. Typing analysis revealed that 63, 3, 1, 4, 1 and 2 cases harboring type16, 18, 31, 35, 58 and plural, respectively.
In conclusion, HPV was detected in 62% of Japanese OC cases, of which type 16 was by far the most frequent (85%). Ectopic chromosome around centrosome (ECAC) was detected in 69% of HPV-positive cases but never in HPV-negative cases. The ECAC was thus a distinct marker for the high risk HPV association of OCs.

Free Research Field

病理