Development of biosensing and bioremediation system for bisphenol using bacterial chemotaxis

2015 Fiscal Year Final Research Report

• Project/Area Number: 24510091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Utsunomiya University
• Principal Investigator: Toshiyuki Nikata 宇都宮大学, 工学(系)研究科(研究院), 助教 (30272222)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 走化性 / Stenotrophomonas / maltophilia / クロロフェノール

Outline of Final Research Achievements

Stenotrophomonas maltophilia is a motile bacterium which accumulates to many kinds of phenols especially bisphenol A. This chemotactic response is mediated by transmembrane chemoreceptor proteins. S. maltophilia harbors putative twelve chemoreceptor genes and it is important to characterize these chemoreceptors for developing novel chemical detecting system or efficient bioremediation technique. In this study, we revealed that chemoreceptor Mcp5 and Mcp6 mainly mediated chemotaxis towards phenol and p-chlorophenol. We also found that this bacterium showed chemotactic response toward halogenized phenol such as p-boromophenol and p-iodophenol which were recognized by chemoreceptor Mcp1.These findings are useful for obtaining all-encompassing chemotaxis profile of S. maltophilia.

Free Research Field

生物環境工学