Fluxomic approaches for Yeast metabolism

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Keio University
• Principal Investigator: MURRAY Douglas 慶應義塾大学, 政策・メディア研究科(藤沢), 特任准教授 (50458965)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 国際研究者交流 / ドイツ:オーストリア:英 国 / 計算および実験結果の交換

Outline of Final Research Achievements

Eukaryotic metabolism is a complex and highly dynamic process that can have a huge impact on the physiology of the organism. While we understand much about the major systems in metabolism a holistic view of how metabolic events impact metabolism remain elusive. In this project we developed experimental protocols, bioinformatic pipelines and computational tools to assist our understanding of how cellular respiration and bioenergetics influence metabolism. In particular, we concentrated on the the calculation and estimation of pathway fluxes. We applied these methods to precisely controlled continuous cultures of bakers' yeast to reveal nucleotide biosynthesis, large-scale mobilisation of lipids and carbon dioxide assimilation occurs during high respiration states. Currently, we are validating and integrating these data and plan to publish our findings in the public domain soon.

Free Research Field

Systems Biology