Mechanism of lifespan regulation in diploid cells of the budding yeast Saccharomyces cerevisiae as a model for cellular lifespan study

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: MUKAI Yukio 長浜バイオ大学, バイオサイエンス学部, 准教授 (60252615)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 寿命 / 二倍体細胞 / 出芽酵母 / 老化疾患モデル / トランスクリプトーム / メタボローム / 必須遺伝子

Outline of Final Research Achievements

The budding yeast Saccharomyces cerevisiae is one of the most important model organisms for studying cellular senescence and lifespan. Haploid cells of the yeast is convenient for analyzing gene function. In this study, we have investigated yeast diploid cells as a better model for cellular lifespan study since higher organisms are generally diploid cells. Diploid cells of wild-type strain exhibited longer replicative lifespan, the number of cells produced from a cell before dying, than haploid cells. During searching genes that are specifically expressed in diploid cells, we found novel longevity genes. Using heterozygous diploid knockout mutants, we discovered novel longevity genes essential for cell proliferation.

Free Research Field

酵母の分子遺伝学