Development of single nanoparticle measurement system for analysis of uptake behavior of nanoparticles in biological cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: INAGAKI KAZUMI 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究室長 (50356490)
• Co-Investigator(Kenkyū-buntansha): TAKATSU Akiko 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究科長 (10357361) ; FUJII Shin-ichirou 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (10415739) ; MIYASHITA Shin-ichi 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究員 (60614766)
• Co-Investigator(Renkei-kenkyūsha): KATO Haruhisa 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (10462839)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ナノ粒子 / 細胞 / 質量分析

Outline of Final Research Achievements

A single nanoparticle measurement system using ICP-MS was developed for analysis of uptake and dissolution behavior of nanoparticles in biological cells. In this study, a high speed detection system that can measure with time resolution less than 10 us was built in ICP-MS instrument, and an effective data processing scheme was established. Furthermore, direct cell introduction system was modified for extending a capability for introducing a large cells. For checking the performance of the system, the system developed was applied for evaluating uptake and dissolution behavior of nanoparticles in microalgal cells.

Free Research Field

分析化学