Development of a new method for chemical synthesis of genomic DNAs

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: AKIHIRO Ohkubo 東京工業大学, 生命理工学研究科, 准教授 (60376960)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: DNA合成 / ホスホロアミダイト / ポーラスガラス

Outline of Final Research Achievements

The final goal of this study is development of a high-throughput method for chemical synthesis of the genomic DNAs of mycoplasma and cyanobacteria. In this research, ‘improvement of chain elongation efficiency’, ‘simplification of purification of oligonucleotides’, and ‘development of a flow-system for synthesis of long oligonucleotides’ was carried out. Specifically, l the 30-mer DNA oligomers was synthesized by using the condensation reaction on the activator-contained solid phase carrier and a capping reaction with diphenylphosphonate. We also prepared a filter-type porous glass carrier for the flow system of DNA synthesis and examined the reactivity on the support in detail.

Free Research Field

生体機能関連化学