Study on the controlling mechanism of fibrin gel formation by the unordered region of fibrinogen and N-linked saccharide chain

2014 Fiscal Year Final Research Report

• Project/Area Number: 24550245
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Polymer/Textile materials
• Research Institution: Gunma University
• Principal Investigator: KUBOTA Kenji 群馬大学, 大学院理工学府, 教授 (40153332)
• Co-Investigator(Kenkyū-buntansha): NAMEKI Nobukazu 群馬大学, 大学院理工学府, 准教授 (80302959)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ゲル / 生体高分子 / フィブリノゲン / Bβ鎖N末端領域 / ラテラル凝集 / Fragment-D / 表面プラズモン共鳴

Outline of Final Research Achievements

The following schemes in the fibrin gel formation process were strongly suggested by the SPR measurements using recombinant (and mutated) fibrinogen fragment corresponding to the N-terminal region of fibrinogen Bβ chain: 66 amino acid residues in the N-terminal region of fibrinogen Bβpolypeptide chain (BβN) play an essential role in the fibrin gel formation through interacting with the distal D-regions of fibrinogen. The central factor in that interaction is the unordered region with high mobility around Bβ44R, especially Bβ44R contributes notably to the interaction. N-linked carbohydrate chain inhibitingly affects the interaction between BβN and D-region, thus suppresses the lateral aggregation of protofibrils.

Free Research Field

化学