2014 Fiscal Year Final Research Report
Studies of the low-cost immobilization system for recombinant proteins using beta-GRP and curdlan.
| Project/Area Number |
24560954
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Health Sciences University of Hokkaido (2013-2014)
Hokkaido University (2012) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | タンパク質 / バイオテクノロジー / バイオリアクター / 生物・生体工学 / ナノ材料 |
|---|
| Outline of Final Research Achievements |
Amino terminal domain of Silkworm βGRP (GRP-tag) tightly and specifically associates with a water-insoluble β-1,3-glucan, curdlan. We have studied a novel immobilization system for recombinant proteins based on the association between the GRP-tag and curdlan. The recombinant proteins fused to the GRP-tag were expressed in several hosts and were immobilized in one step on the curdlan beads from cell lysates and medium. Furthermore, we succeeded in preparation of a curdlan disk and curdlan sheet as a support carrier for GRP-tag.
|
| Free Research Field |
生物工学
|
