Screening of bioactive peptide from marine metagenome and specific amplification methods for potential genes for drug discovery

2014 Fiscal Year Final Research Report

• Project/Area Number: 24560964
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Hiroshima University
• Principal Investigator: OKAMURA YOSHIKO 広島大学, 先端物質科学研究科, 准教授 (80405513)
• Co-Investigator(Kenkyū-buntansha): SAITO Yumiko 広島大学, 大学院総合科学研究科, 教授 (00215568)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メタゲノム / GPCR / 創薬ターゲット

Outline of Final Research Achievements

The methods for drug screening using genomic resources from VBNC (viable but non-culturable) bacteria were developed. The surrogate ligand of orphan GPCR was screened among metagenomic library and we successfully found the candidate and its gene cluster. This result suggests that the gene resources from VBNC bacteria would be applicable to drug discvery. Moreover, we established the method that the whole genome harboring target genes for drug discovery were amplified specifically among the majority of non-target.

Free Research Field

微生物ゲノム工学