2014 Fiscal Year Final Research Report
Molecular mechanism for the repair of photosynthesis from light-induced damage
Project/Area Number |
24570039
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Saitama University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Yasuhiro 埼玉大学, 大学院理工学研究科, 教授 (10154874)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | 光合成 / ストレス / タンパク質合成 / 翻訳因子 |
Outline of Final Research Achievements |
We have previously reported that reactive oxygen species (ROS) act primarily by inhibiting the repair of photosystem II (PSII) during photoinhibition; that ROS suppress the synthesis de novo of proteins that are necessary for the repair of PSII; and that translation factor EF-G is a target of ROS. In the present study, we found that another translation factor EF-Tu is also a target of ROS, being inactivated via formation of an intermolecular disulfide bond and sulfenic acid at Cys82 in the cyanobacterium Synechocystis sp PCC 6803; that expression of EF-Tu in which Cys82 had been replaced by serine rendered Synechocystis cells more resistant against photoinhibition of PSII; and that chloroplast-localized EF-G and EF-Tu from Arabidopsis thaliana are also sensitive to oxidation by ROS.
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Free Research Field |
植物分子生理学
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