Study for coordinated growth by epidermal cell layer in Arabidopsis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KURATA Tetsuya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (50360540)
• Co-Investigator(Renkei-kenkyūsha): SAKAMOTO Tomoaki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 博士研究員 (10600132)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: シロイヌナズナ / 器官成長 / 表皮細胞 / RNA-sequencing / 協調化

Outline of Final Research Achievements

Organ growth of multicellular plant is coordinated among cells/tissues. Old and recent physiological studies showed the outer-most cell layer, epidermis, have restrictive and promotive effects on organ growth in plants.
In this study, we defined the genes whose expression were up-regulated in the epidermis layers and under the dark condition in Arabidopsis stem and/or hypocotyl tissue. For epidermis-enriched genes, specific cell layers were dissected from paraffin-embedded slices by laser. After linear amplification of small amount RNA from dissected samples, RNA-sequencing was conducted. Informatic analysis revealed that gene expression profiling was appropriate. Combined analysis with our RNA-sequencing and public microarray data defined 73 genes were up-regulated in the epidermis of stem and under the dark condition which enhances cell growth in hypocotyl (juvenile stem-like structure).

Free Research Field

植物分子生物学